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Gene Expression Data
Assay Details
Assay
Reference: J:180770 Bjerknes M, et al., Origin of the brush cell lineage in the mouse intestinal epithelium. Dev Biol. 2012 Feb 15;362(2):194-218
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6741115
Gene symbol: Hes1
Gene name: hes family bHLH transcription factor 1
Antibody: anti-Hes1
Detection system: Secondary antibody coupled to Alexa Fluor 647
Results
Specimen 16A: postnatal (more )
Note: Double labeled: orange - Hes1; red - Gfi1b (assay MGI:6741054).
Structure Level Pattern Image Note
TS28: crypt of lieberkuhn Present 16A-3 , 16A-1 No expression in the nuclei of most brush lineage cells.
TS28: intestine epithelium Present 16A-3 , 16A-1 No expression in the nuclei of most brush lineage cells.

Specimen 16B: postnatal (more )
Note: Double labeled: orange - Hes1; green - Gfi1b (assay MGI:6741116).
Structure Level Pattern Image Note
TS28: crypt of lieberkuhn Weak 16B-3 , 16B-1 No expression in the nuclei of most brush lineage cells. Only the small, immature cell in the crypt base was weakly positive.
TS28: intestine epithelium Weak 16B-3 , 16B-1 No expression in the nuclei of most brush lineage cells. Only the small, immature cell in the crypt base was weakly positive.

Specimen 16C: postnatal (more )
Note: Double labeled: orange - Hes1; red - Gfi1b (assay MGI:6741054).
Structure Level Pattern Image Note
TS28: crypt of lieberkuhn Present 16C-1 , 16C-3 No expression in the nuclei of most brush lineage cells. Only the small, immature cell in the crypt base was weakly positive.
TS28: intestine epithelium Present 16C-1 , 16C-3 No expression in the nuclei of most brush lineage cells. Only the small, immature cell in the crypt base was weakly positive.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/07/2024
MGI 6.23
The Jackson Laboratory