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Gene Expression Data
Assay Details
Assay
Reference: J:303267 Singh E, et al., Dynamic changes in endoglin expression in the developing mouse heart. Gene Expr Patterns. 2021 Mar;39:119165
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6730262
Gene symbol: Pecam1
Gene name: platelet/endothelial cell adhesion molecule 1
Antibody: MEC 13.3
Results
Specimen 1A: embryonic day 11.5 (more )
Note: Double labeled: red - CD31 (Pecam1); green - Eng (assay MGI:6730259).
Structure Level Pattern Image Note
TS19: left atrium endocardial lining Present 1A CD31 , 1A Merged Expression in atrial and ventricular endocardium, co-expressed with Eng
TS19: left ventricle endocardial lining Present 1A CD31 , 1A Merged Expression in atrial and ventricular endocardium, co-expressed with Eng
TS19: right atrium endocardial lining Present 1A CD31 , 1A Merged Expression in atrial and ventricular endocardium, co-expressed with Eng
TS19: right ventricle endocardial lining Present 1A CD31 , 1A Merged Expression in atrial and ventricular endocardium, co-expressed with Eng
Specimen 1B: embryonic day 11.5 (more )
Note: Double labeled: red - CD31 (Pecam1); green - Eng (assay MGI:6730259).
Structure Level Pattern Image Note
TS19: ventricle endocardial lining Present 1B CD31 , 1B Merged
TS19: visceral pericardium Absent 1B Merged , 1B CD31 No expression in epicardial cells.
Specimen 2A: embryonic day 13.5 (more )
Note: Double labeled: red - CD31 (Pecam1); green - Eng (assay MGI:6730259).
Structure Level Pattern Image Note
TS21: left atrium endocardial lining Present Widespread 2A Merged , 2A CD31 Expression throughout atrial and ventricular endocardium, co-expressed with Eng.
TS21: left ventricle endocardial lining Present Widespread 2A Merged , 2A CD31 Expression throughout atrial and ventricular endocardium, co-expressed with Eng.
TS21: right atrium endocardial lining Present Widespread 2A Merged , 2A CD31 Expression throughout atrial and ventricular endocardium, co-expressed with Eng.
TS21: right ventricle endocardial lining Present Widespread 2A Merged , 2A CD31 Expression throughout atrial and ventricular endocardium, co-expressed with Eng.
Specimen 2B: embryonic day 13.5 (more )
Note: Double labeled: red - CD31 (Pecam1); green - Eng (assay MGI:6730259).
Structure Level Pattern Image Note
TS21: ventricle endocardial lining Present 2B Merged , 2B CD31
Specimen 2B inset: embryonic day 13.5 (more )
Note: Double labeled: red - CD31 (Pecam1); green - Eng (assay MGI:6730259).
Structure Level Pattern Image Note
TS21: myocardium layer Absent 2B Merged inset , 2B CD31 inset No expression in some cells in myocardium, likely corresponding to epicardially derived cells (EPDCs).
Specimen 3A: embryonic day 15.5 (more )
Note: Double labeled: red - CD31 (Pecam1); green - Eng (assay MGI:6730259).
Structure Level Pattern Image Note
TS23: left atrium endocardial lining Present 3A CD31 , 3A Merged Expression in atrial and ventricular endocardium, and in sub-epicardial blood vessels, co-expressed with Eng.
TS23: left ventricle endocardial lining Present 3A CD31 , 3A Merged Expression in atrial and ventricular endocardium, and in sub-epicardial blood vessels, co-expressed with Eng.
TS23: right atrium endocardial lining Present 3A CD31 , 3A Merged Expression in atrial and ventricular endocardium, and in sub-epicardial blood vessels, co-expressed with Eng.
TS23: right ventricle endocardial lining Present 3A CD31 , 3A Merged Expression in atrial and ventricular endocardium, and in sub-epicardial blood vessels, co-expressed with Eng.
TS23: heart blood vessel Present Regionally restricted 3A CD31 , 3A Merged Expression in sub-epicardial blood vessels.v
Specimen 3B: embryonic day 15.5 (more )
Note: Double labeled: red - CD31 (Pecam1); green - Eng (assay MGI:6730259).
Structure Level Pattern Image Note
TS23: ventricle endocardial lining Present 3B CD31 , 3B Merged
TS23: heart blood vessel Present Regionally restricted 3B Merged , 3B CD31 Expression in in sub-epicardial blood vessels,
Specimen 3B inset: embryonic day 15.5 (more )
Note: Double labeled: red - CD31 (Pecam1); green - Eng (assay MGI:6730259).
Structure Level Pattern Image Note
TS23: visceral pericardium Absent 3B Merged inset , 3B CD31 inset No expression in epicardially derived cells (EPDCs).
Specimen 6A: embryonic day 13.5 (more )
Note: Double labeled: red - CD31 (Pecam1); green - Eng (assay MGI:6730259).
Structure Level Pattern Image Note
TS21: tricuspid valve Present Regionally restricted 6A CD31 , 6A Merged Expression in valve endocardial cells. No expression in valve mesenchyme.
Specimen 6B: embryonic day 15.5 (more )
Note: Double labeled: red - CD31 (Pecam1); green - Eng (assay MGI:6730259).
Structure Level Pattern Image Note
TS23: tricuspid valve Present Regionally restricted 6B Merged , 6B CD31 Expression in valve endocardial cells. No expression in valve mesenchyme.
Specimen 6C: embryonic day 17.5 (more )
Note: Double labeled: red - CD31 (Pecam1); green - Eng (assay MGI:6730259).
Structure Level Pattern Image Note
TS25: tricuspid valve Present Regionally restricted 6C Merged , 6C CD31 Expression in valve endocardial cells. No expression in valve mesenchyme.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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