Sycp1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:278517 Li M, et al., The histone modification reader ZCWPW1 is required for meiosis prophase I in male but not in female mice. Sci Adv. 2019;5(8):eaax1101
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6727332
Gene symbol:	Sycp1
Gene name:	synaptonemal complex protein 1

Antibody:	anti-SYCP1
Detection system:	Alexa Fluor 488
Assay notes:	Multiple Sycp1 antibodies were described in this paper, but the one used in this assay was not detailed. Sycp1 antibodies MGI:6431090 or MGI:6431090 may have been used in this assay.

Results

3A ©

Specimen 3A: postnatal day 35 (more

)
Note: Leptotene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Absent		3A	Absence of expression indicates central element (CE) of synaptonemal complex was not yet present.

3B ©

Specimen 3B: postnatal day 35 (more

)
Note: Zygotene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Trace	Regionally restricted	3B	Expression in central element (CE) of synaptonemal complex.

3C ©

Specimen 3C: postnatal day 35 (more

)
Note: Pachytene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present	Regionally restricted	3C	Continuous expression in pachytene spermatocytes at full synapsis on autosomes.

3D ©

Specimen 3D: postnatal day 35; Zcwpw1^em1Kliu/Zcwpw1^em1Kliu (more

)
Note: Leptotene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Absent		3D

3E ©

Specimen 3E: postnatal day 35; Zcwpw1^em1Kliu/Zcwpw1^em1Kliu (more

)
Note: Zygotene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Trace	Regionally restricted	3E	Expression beginning to appear on paired chromosomes, indicating synapsis somewhat initiated.

3F ©

Specimen 3F: postnatal day 35; Zcwpw1^em1Kliu/Zcwpw1^em1Kliu (more

)
Note: Pachytene-like. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present	Regionally restricted	3F	Large number of chromosomes remain unsynapsed.

S3A ©

Specimen S3A: postnatal; Zcwpw1^em1Kliu/Zcwpw1^em1Kliu (more

)
Note: Leptotene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Absent		S3A

S3B ©

Specimen S3B: postnatal; Zcwpw1^em1Kliu/Zcwpw1^em1Kliu (more

)
Note: Early zygotene. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present	Regionally restricted	S3B

S3C ©

Specimen S3C: postnatal; Zcwpw1^em1Kliu/Zcwpw1^em1Kliu (more

)
Note: Late zygotene. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS28: spermatocyte	Present	Regionally restricted	S3C

5C ©

Specimen 5C: embryonic day 17.5 (more

)
Note: Leptotene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS25: oocyte	Absent		5C

5D ©

Specimen 5D: embryonic day 17.5 (more

)
Note: Zygotene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS25: oocyte	Weak		5D

5E ©

Specimen 5E: embryonic day 17.5 (more

)
Note: Pachytene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present	Regionally restricted	5E	Expression in synapsed chromosomes.

5F ©

Specimen 5F: embryonic day 17.5; Zcwpw1^em1Kliu/Zcwpw1^em1Kliu (more

)
Note: Leptotene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS25: oocyte	Absent		5F

5G ©

Specimen 5G: embryonic day 17.5; Zcwpw1^em1Kliu/Zcwpw1^em1Kliu (more

)
Note: Zygotene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS25: oocyte	Weak		5G

5H ©

Specimen 5H: embryonic day 17.5; Zcwpw1^em1Kliu/Zcwpw1^em1Kliu (more

)
Note: Pachytene stage. Double labeled: green - Sycp1; red - Sycp3 (assay MGI:6727331).

Structure	Level	Pattern	Image	Note
TS25: oocyte	Present	Regionally restricted	5H	Expression in synapsed chromosomes.

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