Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:272319
Tiveron MC, et al., Expression and function of CXCR7 in the mouse forebrain. J Neuroimmunol. 2010;224(1-2):72-9
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6715221
|
|
Gene symbol:
|
Ackr3
|
|
Gene name:
|
atypical chemokine receptor 3
|
|
Probe:
|
Cxcr7 probe2
|
|
Probe preparation:
|
Antisense labelled with fluorescein RNA
|
|
|
Results
|
Specimen
3A MZ:
embryonic day 15.5
(more  )
Note: Double labeled: green - Ackr3; red - Lhx6 (assay MGI:6715240).
Specimen
3A MZ:
(close  )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Ackr3; red - Lhx6 (assay MGI:6715240).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex marginal layer |
Present |
Regionally restricted |
3A MZ
|
Ackr3-positive cells in the marginal zone co-expressed Lhx6, a marker of cortical interneurons. Ackr3 was expressed in the majority of interneurons migrating in the marginal zone (73.5%).
|
Specimen
3A SVZ:
embryonic day 15.5
(more )
Note: Double labeled: green - Ackr3; red - Lhx6 (assay MGI:6715240).
Specimen
3A SVZ:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Ackr3; red - Lhx6 (assay MGI:6715240).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex subventricular zone |
Present |
Regionally restricted |
3A SVZ
|
Ackr3-positive cells in the subventricular zone co-expressed Lhx6, a marker of cortical interneurons. Ackr3 was expressed in about 50% of interneurons migrating in the subventricular zone.
|
Specimen
3C:
embryonic day 15.5
(more )
Note: Double labeled: green - Ackr3; red - Cxcr4 (assay MGI:6715238).
Specimen
3C:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Double labeled: green - Ackr3; red - Cxcr4 (assay MGI:6715238).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex intermediate zone |
Present |
Regionally restricted |
3C
|
The majority of interneurons in the subventricular zone/intermediate zone expressed both Ackr3 and Cxcr4 (57.7%), and 21% expressed only Ackr3.
|
| TS23: cerebral cortex subventricular zone |
Present |
Regionally restricted |
3C
|
The majority of interneurons in the subventricular zone/intermediate zone expressed both Ackr3 and Cxcr4 (57.7%), and 21% expressed only Ackr3.
|
Specimen
not shown:
embryonic day 15.5
(more )
Note: Also stained for Cxcr4 (assay MGI:6715238).
Specimen
not shown:
(close )
|
Genetic Background:
|
CD-1
|
|
Age:
|
embryonic day 15.5
|
|
Sex:
|
Not Specified
|
|
Type:
|
section
|
|
Fixation:
|
4% Paraformaldehyde
|
|
Note:
|
Also stained for Cxcr4 (assay MGI:6715238).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS23: cerebral cortex marginal layer |
Present |
Regionally restricted |
|
More than 75% of all interneurons co-expressed Ackr3 and Cxcr4, and 17% expressed Ackr3 alone in the marginal zone.
|
| TS23: cortical plate |
Present |
Regionally restricted |
|
The vast majority of interneurons in the cortical plate expressed both Ackr3 and Cxcr4 (47.5%) or Ackr3 alone (45.1%).
|
|
Analysis Tools