Reporter:	lacZ
	Direct Detection

Structure	Level	Pattern	Image	Note
TS28: afferent arteriole of mature renal corpuscle	Present		1A
TS28: collecting duct	Present		1A
TS28: efferent arteriole of mature renal corpuscle	Present		1A
TS28: glomerular tuft	Present		1A
TS28: interlobular artery	Present		1A
TS28: peritubular capillary	Present		1A
TS28: renal connecting tubule	Present		1A

Structure	Level	Pattern	Image	Note
TS28: renal artery	Present		1B
TS28: smooth muscle layer of renal artery	Present		1B

Structure	Level	Pattern	Image	Note
TS28: outer renal medulla	Present	Regionally restricted	1C	Expression was detected in vascular bundles of the outer medulla.
TS28: collecting duct	Present		1C

Structure	Level	Pattern	Image	Note
TS28: artery smooth muscle	Present		1Ea
TS28: interlobular artery	Present		1Ea

Structure	Level	Pattern	Image	Note
TS28: artery smooth muscle	Present		1Eb
TS28: interlobular artery	Present		1Eb

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Present		1G
TS28: renal connecting tubule	Present		1G
TS28: renal vasculature	Present		1G

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Present		1H
TS28: glomerular tuft	Present		1H
TS28: renal connecting tubule	Present		1H

Structure	Level	Pattern	Image	Note
TS28: loop of Henle distal straight tubule	Absent		1I
TS28: loop of Henle thin limb	Absent		1I
TS28: renal vasculature	Present	Non-Uniform	1I	Vascular bundles are composed of Efnb2-positive and -negative vessels.

Structure	Level	Pattern	Image	Note
TS20: primitive collecting duct	Present	Regionally restricted	2A	Expression was detected in ureteric bud epithelium.
TS20: arterial blood vessel	Present	Regionally restricted	2A	Expression was detected in early arterial vessels entering the metanephros.
TS20: vascular element	Present	Regionally restricted	2A	Expression was detected in the vascular networks surrounding the metanephric blastema and assembled around the ureteric bud.
TS20: dorsal aorta	Present		2A
TS20: mesenchyme of metanephros	Weak		2A

Structure	Level	Pattern	Image	Note
TS21: mesonephric glomerulus	Present	Regionally restricted	2B	Expression was detected in developing glomeruli, including comma-shaped, S-shaped, and capillary loop stage glomeruli.
TS21: renal vasculature	Present	Regionally restricted	2B	Expression was detected in branching arteries.
TS21: collecting duct	Present	Regionally restricted	2B	Expression was detected in ureteric bud.

Structure	Level	Pattern	Image	Note
TS22: mesonephric glomerulus	Present	Regionally restricted	2C	Expression was detected in developing glomeruli, including comma-shaped, S-shaped, and capillary loop stage glomeruli.
TS22: renal vasculature	Present	Regionally restricted	2C	Expression was detected in branching arteries.
TS22: collecting duct	Present	Regionally restricted	2C	Expression was detected in ureteric bud.

Structure	Level	Pattern	Image	Note
TS24: maturing glomerular tuft	Present	Regionally restricted	2D	Expression was detected in developing glomeruli, including comma-shaped, S-shaped, and capillary loop stage glomeruli.
TS24: renal vasculature	Present	Regionally restricted	2D	Expression was detected in arteries and arterioles.
TS24: collecting duct	Present		2D

Structure	Level	Pattern	Image	Note
TS26: maturing glomerular tuft	Present	Regionally restricted	2E	Expression was detected in developing glomeruli, including comma-shaped, S-shaped, and capillary loop stage glomeruli.
TS26: renal vasculature	Present	Regionally restricted	2E	Expression was detected in arteries and arterioles.
TS26: collecting duct	Present		2E

Structure	Level	Pattern	Image	Note
TS26: collecting duct	Present		2E inset	Combined beta-galactosidase staining with DBA staining demonstrated expression in the connecting tubules to be continuous with collecting ducts.
TS26: renal connecting tubule	Present		2E inset	Combined beta-galactosidase staining with DBA staining demonstrated expression in the connecting tubules to be continuous with collecting ducts.

Structure	Level	Pattern	Image	Note
TS28: collecting duct	Present		2F
TS28: renal connecting tubule	Present		2F

Structure	Level	Pattern	Image	Note
TS22: comma-shaped body	Present	Regionally restricted	3Aa	Expression was detected in precursors of the visceral glomerular epithelial cells adjacent to the vascular cleft of comma-shaped glomeruli. Low level expression was detected in endothelial precursors.

Structure	Level	Pattern	Image	Note
TS24: maturing glomerular tuft	Present		3Ab	Prominent expression was detected in glomerular vascular cells, including not only visceral epithelial cells but also endothelial and mesangial cells.
TS24: s-shaped body	Present		3Ab	Prominent expression was detected in glomerular vascular cells, including not only visceral epithelial cells but also endothelial and mesangial cells.

Structure	Level	Pattern	Image	Note
TS24: capillary loop renal corpuscle	Present		3Ac	Prominent expression was detected in glomerular vascular cells, including not only visceral epithelial cells but also endothelial and mesangial cells.
TS24: maturing glomerular tuft	Present		3Ac	Prominent expression was detected in glomerular vascular cells, including not only visceral epithelial cells but also endothelial and mesangial cells.

Structure	Level	Pattern	Image	Note
TS28: visceral epithelium of mature renal corpuscle	Absent		3Ad