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Gene Expression Data
Assay Details
Assay
Reference: J:280280 Rutledge EA, et al., Morphogenesis of the kidney and lung requires branch-tip directed activity of the Adamts18 metalloprotease. Dev Biol. 2019 Oct 15;454(2):156-169
Assay type: RNA in situ
MGI Accession ID: MGI:6512066
Gene symbol: Bmp4
Gene name: bone morphogenetic protein 4
Probe: Mm-Bmp4 (401301)
Probe preparation: Antisense
Visualized with: Chromogenic
Assay notes: RNAscope assay
Results
Specimen 10C: embryonic day 12.5 (more )
Note: Double labeled: red - Bmp4; green - Fgf10 (assay MGI:6512065)
Structure Level Pattern Image Note
TS20: ureteric tip Weak 10C
TS20: ureteric trunk Weak 10C
TS20: metanephros Present 10C Most expression is seen in non-ureteric bud cells.

Specimen 10D: embryonic day 12.5; Adamts18tm1(KOMP)Vlcg/Adamts18tm1(KOMP)Vlcg (more )
Note: Double labeled: red - Bmp4; green - Fgf10 (assay MGI:6512065)
Structure Level Pattern Image Note
TS20: ureteric tip Weak 10D The number of transcripts per cell is the same in the mutant as in wild type.
TS20: ureteric trunk Weak 10D The number of transcripts per cell is the same in the mutant as in wild type.
TS20: metanephros Present 10D The number of transcripts per cell is the same in the mutant as in wild type. Most expression is seen in non-ureteric bud cells.

Specimen 10G: embryonic day 12.5 (more )
Note: Double labeled: red - Bmp4; green - Fgf10 (assay MGI:6512065)
Structure Level Pattern Image Note
TS20: lung epithelium Present Non-Uniform 10G Expression is seen in tip cells; only trace amounts are seen in the stalk.
TS20: lung Present 10G Some expression is seen in non-epithelial cells.

Specimen 10H: embryonic day 12.5; Adamts18tm1(KOMP)Vlcg/Adamts18tm1(KOMP)Vlcg (more )
Note: Double labeled: red - Bmp4; green - Fgf10 (assay MGI:6512065)
Structure Level Pattern Image Note
TS20: lung epithelium Present Non-Uniform 10H Expression is seen in tip cells; only trace amounts are seen in the stalk. The number of transcripts per cell is the same in the mutant as in wild type.
TS20: lung Present 10H Some expression is seen in non-epithelial cells. The number of transcripts per cell is the same in the mutant as in wild type.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
03/25/2025
MGI 6.24
The Jackson Laboratory