Antibody:	anti-ChAT (AB144P)
Detection system:	Secondary antibody

Structure	Level	Pattern	Image	Note
TS27: telencephalon septum	Present	Regionally restricted	1c1	Expression is present in septal neurons.

Structure	Level	Pattern	Image	Note
TS27: hippocampus CA1	Present	Regionally restricted	1c2	Expression is present in neurons.

Structure	Level	Pattern	Image	Note
TS27: hippocampus CA1	Present	Regionally restricted	1c3	Expression is present in neurons.

Structure	Level	Pattern	Image	Note
TS27: hippocampus CA1	Present	Regionally restricted	1c4	Expression is present in neurons.

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Present	Regionally restricted	2a right , 2a center	Expression is present in synapses that are organized into perisomatic cholinergic "baskets."

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Present	Regionally restricted	2a1 right , 2a1 center	Expression is present in synapses that are organized into perisomatic cholinergic "baskets"; although the number of these synapses does not decrease in the mutant, the baskets are disordered and their number is reduced.

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Present	Regionally restricted	2a2 wild-type left , 2a2 wild-type right	Expression is present in synapses that are organized into perisomatic cholinergic "baskets."

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Present	Regionally restricted	2a2 DAGLa KO right , 2a2 DAGLa KO left	Expression is present in synapses that are organized into perisomatic cholinergic "baskets"; although the number of these synapses does not decrease in the mutant, the baskets are disordered and their number is reduced.

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Present	Regionally restricted	2a2 DAGLb KO left , 2a2 DAGLb KO right	Expression is present in synapses that are organized into perisomatic cholinergic "baskets"; although the number of these baskets does not decrease in the mutant, they are disordered.

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Present	Regionally restricted	S1e wild-type right , S1e wild-type left	Expression is present in cholinergic "baskets."

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Present	Regionally restricted	S1e DAGLa-KO right , S1e DAGLa-KO left	Expression is present in cholinergic "baskets"; these baskets are disrupted in the mutant.

Structure	Level	Pattern	Image	Note
TS28: CA1 pyramidal cell layer	Present	Regionally restricted	S1e DAGLb-KO left , S1e DAGLb-KO right	Expression is present in cholinergic "baskets"; these baskets are disrupted in the mutant.