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Gene Expression Data
Assay Details
Assay
Reference: J:283401 Masgutova G, et al., Pou2f2 Regulates the Distribution of Dorsal Interneurons in the Mouse Developing Spinal Cord. Front Mol Neurosci. 2019;12:263
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6491255
Gene symbol: Isl1
Gene name: ISL1 transcription factor, LIM/homeodomain
Antibody: anti-Isl1 (#GT15051)
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Clone 39.4D5 MGI:2149733 may have been used in this assay.
Results
Specimen 3A: embryonic day 10.5 (more )
Note: Double labeled: green - Isl1; red - Pou2f2 (assay MGI:6491252).
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3A Expression in dl3 interneurons.
Specimen 3B: embryonic day 10.5; Onecut1tm1Flem/Onecut1tm1Flem, Onecut2tm1Flem/Onecut2tm1Flem (more )
Note: Double labeled: green - Isl1; red - Pou2f2 (assay MGI:6491252).
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 3B Expression in dl3 interneurons.
Specimen 3C: embryonic day 11.5 (more )
Note: Double labeled: green - Isl1; red - Pou2f2 (assay MGI:6491252).
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 3C Expression in dl3 interneurons.
Specimen 3D: embryonic day 11.5; Onecut1tm1Flem/Onecut1tm1Flem, Onecut2tm1Flem/Onecut2tm1Flem (more )
Note: Double labeled: green - Isl1; red - Pou2f2 (assay MGI:6491252).
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 3D Expression in dl3 interneurons.
Specimen 3E: embryonic day 12.5 (more )
Note: Double labeled: green - Isl1; red - Pou2f2 (assay MGI:6491252).
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 3E Expression in dl3 interneurons.
Specimen 3F: embryonic day 12.5; Onecut1tm1Flem/Onecut1tm1Flem, Onecut2tm1Flem/Onecut2tm1Flem (more )
Note: Double labeled: green - Isl1; red - Pou2f2 (assay MGI:6491252).
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 3F Expression in dl3 interneurons.
Specimen 6D: embryonic day 10.5 (more )
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 6D Expression in dl3 interneurons.
Specimen 6E: embryonic day 10.5; Pou2f2tm1Bal/Pou2f2tm1Bal (more )
Structure Level Pattern Image Note
TS17: future spinal cord Present Regionally restricted 6E Expression in dl3 interneurons.
Specimen 8A: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 8A Expression in dl3 interneurons.
Specimen 8B: embryonic day 12.5; Pou2f2tm1Bal/Pou2f2tm1Bal (more )
Structure Level Pattern Image Note
TS20: spinal cord Present Regionally restricted 8B Expression in dl3 interneurons.
Specimen 8M: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 8M Expression in dl3 interneurons.
Specimen 8N: embryonic day 14.5; Pou2f2tm1Bal/Pou2f2tm1Bal (more )
Structure Level Pattern Image Note
TS22: spinal cord Present Regionally restricted 8N
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory