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Gene Expression Data
Assay Details
Assay
Reference: J:266577 Rodrigo Albors A, et al., Lineage tracing of axial progenitors using Nkx1-2CreER(T2) mice defines their trunk and tail contributions. Development. 2018 Oct 2;145(19):dev164319
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6471021
Gene symbol: T
Gene name: brachyury, T-box transcription factor T
Antibody: anti-Brachyury (AF2085)
Detection system: Secondary antibody coupled to Alexa Fluor
Results
Specimen 2A rostral node: embryonic day 8.5 (more )
Note: Double labeled: red - T; blue - Sox2 (assay MGI:6471020).
Structure Level Pattern Image Note
TS13: embryo ectoderm Present Regionally restricted 2A rostral node T , 2A rostral node (merge) Expression higher in midline epiblast and primitive streak than in CLE (caudal lateral epiblast).
Specimen 2A NSB: embryonic day 8.5 (more )
Note: Double labeled: red - T; blue - Sox2 (assay MGI:6471020).
Structure Level Pattern Image Note
TS13: embryo ectoderm Present Regionally restricted 2A NSB T , 2A NSB (merge) Co-expression with Sox2 in cells at midline of epiblast of NSB (node-streak border).
Specimen 2A streak and CLE: embryonic day 8.5 (more )
Note: Double labeled: red - T; blue - Sox2 (assay MGI:6471020).
Structure Level Pattern Image Note
TS13: embryo ectoderm Present Regionally restricted 2A streak and CLE (merge) , 2A streak and CLE T Co-expression with Sox2 posteriorly in CLE (caudal lateral epiblast).
TS13: primitive streak Present Regionally restricted 2A streak and CLE T , 2A streak and CLE (merge) Co-expression with Sox2 posteriorly in primitive streak.
Specimen 2B (1st row): embryonic day 10.5 (more )
Note: Double labeled: red - T; blue - Sox2 (assay MGI:6471020).
Structure Level Pattern Image Note
TS17: embryo mesenchyme Present Regionally restricted 2B 1st row (merge) , 2B 1st row T Expression in cells in mesenchymal cells directly below neural tube.
Specimen 2B (2nd row): embryonic day 10.5 (more )
Note: Double labeled: red - T; blue - Sox2 (assay MGI:6471020).
Structure Level Pattern Image Note
TS17: embryo mesenchyme Present Regionally restricted 2B 2nd row (merge) , 2B 2nd row T Expression in cells in mesenchymal cells directly below neural tube.
TS17: unsegmented mesenchyme Strong 2B 2nd row T , 2B 2nd row (merge) High levels of expression in presomitic mesoderm cells.
Specimen 2B (3rd row): embryonic day 10.5 (more )
Note: Double labeled: red - T; blue - Sox2 (assay MGI:6471020).
Structure Level Pattern Image Note
TS17: embryo mesenchyme Present Regionally restricted 2B 3rd row T , 2B 3rd row (merge) Expression in putative NMPs (neuromesodermal progenitors) in dorsal half of CNH (chordo-neural hinge), including cells in mesenchymal cells directly below neural tube.
Specimen 2B (4th row): embryonic day 10.5 (more )
Note: Double labeled: red - T; blue - Sox2 (assay MGI:6471020).
Structure Level Pattern Image Note
TS17: tail mesenchyme Present 2B 4th row T , 2B 4th row (merge)
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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