Shh Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:292838 Iulianella A, et al., The Hedgehog receptor Patched1 regulates proliferation, neurogenesis, and axon guidance in the embryonic spinal cord. Mech Dev. 2019 Dec;160:103577
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6467286
Gene symbol:	Shh
Gene name:	sonic hedgehog

Antibody:

5E1

Results

1D ©

Specimen 1D: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: future hindbrain floor plate	Present		1D
TS15: notochord	Present		1D

1E ©

Specimen 1E: embryonic day 9.5; Ptch1^wig/Ptch1^wig (more

)

Structure	Level	Pattern	Image	Note
TS15: future hindbrain floor plate	Present	Regionally restricted	1E	Expression expanded from floor plate, especially in more anterior regions of neural tube.
TS15: notochord	Present		1E

1H ©

Specimen 1H: embryonic day 9.5 (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present		1H
TS15: notochord	Present		1H

1I ©

Specimen 1I: embryonic day 9.5; Ptch1^wig/Ptch1^wig (more

)

Structure	Level	Pattern	Image	Note
TS15: neural tube floor plate	Present	Regionally restricted	1I	Expression expanded from floor plate.

5A ©

Specimen 5A: embryonic day 10.5 (more

)
Note: Triple labeled: green - Shh; red - Tag-1 (Cntn2 assay MGI:6467295); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		5A

5B ©

Specimen 5B: embryonic day 10.5; Ptch1^wig/Ptch1^wig (more

)
Note: Triple labeled: green - Shh; red - Tag-1 (Cntn2 assay MGI:6467295); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	5B	Authors report aberrant dorsal exit of ventral motor roots and reduced crossing of commissural fibers in ventral commissure.

5C ©

Specimen 5C: embryonic day 10.5 (more

)
Note: Triple labeled: green - Shh; red - Tag-1 (Cntn2 assay MGI:6467295); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present		5C

5D ©

Specimen 5D: embryonic day 10.5; Ptch1^wig/Ptch1^wig (more

)
Note: Triple labeled: green - Shh; red - Tag-1 (Cntn2 assay MGI:6467295); blue - DAPI.

Structure	Level	Pattern	Image	Note
TS17: neural tube	Present	Regionally restricted	5D	Aberrant targeting of commissural axons towards floorplate. Dorsal exit of ventral root.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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