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Gene Expression Data
Assay Details
Assay
Reference: J:228856 DiTommaso T, et al., Keratin 76 is required for tight junction function and maintenance of the skin barrier. PLoS Genet. 2014 Oct;10(10):e1004706
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6456583
Gene symbol: Krt76
Gene name: keratin 76
Antibody: anti-Krt76 (HPA019696)
Assay notes: Two antibodies were described, but the one used in this assay was not detailed. Krt76 antibody MGI:6456163 may have been used in this assay.
Results
Specimen 1A KRT76: postnatal adult (more )
Note: Triple labeled: green - Krt76; red - Krt14 (assay MGI:6456584); blue - DAPI.
Structure Level Pattern Image Note
TS28: metapodial pad epidermis Present Regionally restricted 1K KRT76/KRT14 Highest expression in granular layer overlapping with Flg expression.
Specimen 1L +/+: postnatal adult (more )
Note: Triple labeled: green - Krt76; red - Krt14 (assay MGI:6456584); blue - DAPI.
Structure Level Pattern Image Note
TS28: epidermis granular layer Present Regionally restricted 1L +/+ Expression in the granular layer.
Specimen 1L tm1a/tm1a: postnatal adult; Krt76tm1a(KOMP)Wtsi/Krt76tm1a(KOMP)Wtsi (more )
Note: Triple labeled: green - Krt76; red - Krt14 (assay MGI:6456584); blue - DAPI.
Structure Level Pattern Image Note
TS28: epidermis granular layer Absent 1L tm1a/tm1a Granular layer expression was absent.
Specimen 1N E14.5: embryonic day 14.5 (more )
Note: Triple labeled: green - Krt76; red - Krt14 (assay MGI:6456584); blue - DAPI.
Structure Level Pattern Image Note
TS22: back skin Present 1N E14.5
Specimen 1N E18.5: embryonic day 18.5 (more )
Note: Triple labeled: green - Krt76; red - Krt14 (assay MGI:6456584); blue - DAPI.
Structure Level Pattern Image Note
TS26: back skin Strong 1N E18.5 Increasing levels of expression associated with differentiation of skin during later development.
Specimen 1N P1: postnatal day 1 (more )
Note: Triple labeled: green - Krt76; red - Krt14 (assay MGI:6456584); blue - DAPI.
Structure Level Pattern Image Note
TS27: back skin Present 1N P1 Subsequent reduction in expression levels after birth.
Specimen 1N Adult: postnatal adult (more )
Note: Triple labeled: green - Krt76; red - Krt14 (assay MGI:6456584); blue - DAPI.
Structure Level Pattern Image Note
TS28: epidermis granular layer Weak Regionally restricted 1N Adult Low levels of expression in spinous and granular cell layers in adult.
TS28: epidermis spinous layer Weak Regionally restricted 1N Adult Low levels of expression in spinous and granular cell layers in adult.
Specimen 1N': postnatal adult; Krt76tm1a(KOMP)Wtsi/Krt76tm1a(KOMP)Wtsi (more )
Note: Triple labeled: green - Krt76; red - Krt14 (assay MGI:6456584); blue - DAPI.
Structure Level Pattern Image Note
TS28: back skin Absent 1N'
Specimen 2F (No wound): postnatal adult (more )
Note: Triple labeled: green - Krt76; red - Krt14 (assay MGI:6456584); blue - DAPI.
Structure Level Pattern Image Note
TS28: back skin Ambiguous 2F (No wound)
Specimen S2A +/+: postnatal adult (more )
Note: Triple labeled: green - Krt76; red - ZO-1 (Tjp1; assay MGI:6456598); blue - DAPI.
Structure Level Pattern Image Note
TS28: metapodial pad Present S2A +/+
Specimen S2A tm1a/tm1a: postnatal adult; Krt76tm1a(KOMP)Wtsi/Krt76tm1a(KOMP)Wtsi (more )
Note: Triple labeled: green - Krt76; red - ZO-1 (Tjp1; assay MGI:6456598); blue - DAPI.
Structure Level Pattern Image Note
TS28: metapodial pad epidermis Present S2A tm1a/tm1a
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
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