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Gene Expression Data
Assay Details
Assay
Reference: J:148710 Ezhkova E, et al., Ezh2 orchestrates gene expression for the stepwise differentiation of tissue-specific stem cells. Cell. 2009 Mar 20;136(6):1122-35
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6450680
Gene symbol: Ezh2
Gene name: enhancer of zeste 2 polycomb repressive complex 2 subunit
Antibody: anti-Ezh2
Results
Specimen 1B: embryonic day 14.0 (more )
Note: Double labeled: red - Ezh2; green - Itgb4 (assay MGI:6450681).
Structure Level Pattern Image Note
TS22: epidermis Strong Regionally restricted 1B Expression in basal layer and downregulated in suprabasale.
Specimen 1C: embryonic day 18.0 (more )
Note: Double labeled: red - Ezh2; green - Itgb4 (assay MGI:6450681).
Structure Level Pattern Image Note
TS26: epidermis basal layer Strong 1C
TS26: epidermis cornified layer Weak 1C
TS26: epidermis granular layer Weak 1C
TS26: epidermis spinous layer Weak 1C
Specimen 1D: postnatal day 9 (more )
Note: Double labeled: red - Ezh2; green - Itgb4 (assay MGI:6450681).
Structure Level Pattern Image Note
TS28: epidermis basal layer Trace 1D Authors report age-related decline in basal expression. By P9, barely detected even in basal cells.
Specimen 1F E14: embryonic day 14.0 (more )
Note: Double labeled: red - Ezh2; green - BrdU.
Structure Level Pattern Image Note
TS22: epidermis Present 1F E14
Specimen 1F E18: embryonic day 18.0 (more )
Note: Double labeled: red - Ezh2; green - BrdU.
Structure Level Pattern Image Note
TS26: epidermis basal layer Weak 1F E18 Age-related decline in basal expression correlates with reduced proliferative potential.
Specimen 1F P9: postnatal day 9 (more )
Note: Double labeled: red - Ezh2; green - BrdU.
Structure Level Pattern Image Note
TS28: epidermis basal layer Trace 1F P9 Few expressing cells also positive for BrdU.
Specimen 3A WT: postnatal day 0 (more )
Note: Double labeled: red - Ezh2; green - Itgb4 (assay MGI:6450681).
Structure Level Pattern Image Note
TS27: epidermis basal layer Present 3A WT
Specimen 3A cKO: postnatal day 0; Ezh2tm1Tara/Ezh2tm1Tara, Tg(KRT14-cre)1Efu/0 (more )
Note: Conditional mutant. Double labeled: red - Ezh2; green - Itgb4 (assay MGI:6450681).
Structure Level Pattern Image Note
TS27: epidermis basal layer Absent 3A cKO
Specimen S1I: postnatal day 9 (more )
Note: Double labeled: red - Ezh2; green - Itgb4 (assay MGI:6450681).
Structure Level Pattern Image Note
TS28: hair outer root sheath Present S1I
Specimen S1J: postnatal day 22 (more )
Note: Double labeled: red - Ezh2; green - Krt5 (assay MGI:6450683).
Structure Level Pattern Image Note
TS28: hair root sheath matrix Present S1J
Specimen S1L: postnatal day 22 (more )
Note: Double labeled: red - Ezh2; green - Cd34 (assay MGI:6450686).
Structure Level Pattern Image Note
TS28: hair follicle bulge Absent S1L
Specimen S4A WT: embryonic day 16.0 (more )
Note: Double labeled: red - Ezh2; green - Itgb4 (assay MGI:6450681).
Structure Level Pattern Image Note
TS24: epidermis Present Regionally restricted S4A WT Expression in basal cells.
Specimen S4A cKO: embryonic day 16.0; Ezh2tm1Tara/Ezh2tm1Tara, Tg(KRT14-cre)1Efu/0 (more )
Note: Conditional mutant. Double labeled: red - Ezh2; green - Itgb4 (assay MGI:6450681).
Structure Level Pattern Image Note
TS24: epidermis Absent S4A cKO
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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Send questions and comments to User Support. 		last database update
05/28/2024
MGI 6.13 		The Jackson Laboratory