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Gene Expression Data
Assay Details
Assay
Reference: J:287264 Hael CE, et al., The transcriptional regulator PRDM12 is critical for Pomc expression in the mouse hypothalamus and controlling food intake, adiposity, and body weight. Mol Metab. 2020 Apr;34:43-53
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6436934
Gene symbol: Pomc
Gene name: pro-opiomelanocortin-alpha
Antibody: anti-ACTH
Detection system: Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase
Results
Specimen 2B +/-: embryonic day 13.5; Prdm12tm2b(EUCOMM)Hmgu/Prdm12+ (more )
Note: Double labeled: brown - Pomc; blue - Prdm12 (assay MGI:6436933)
Structure Level Pattern Image Note
TS21: hypothalamus Present Regionally restricted 2B +/- , 2B +/- inset Staining is prominent in the basal hypothalamus (future arcuate nucleus).
TS21: pituitary gland Present 2B +/- inset , 2B +/- Expression is seen in Rathke's pouch.
Specimen 2B -/-: embryonic day 13.5; Prdm12tm2b(EUCOMM)Hmgu/Prdm12tm2b(EUCOMM)Hmgu (more )
Note: Double labeled: brown - Pomc; blue - Prdm12 (assay MGI:6436933)
Structure Level Pattern Image Note
TS21: hypothalamus Trace 2B -/- inset , 2B -/- Staining is negligible/greatly diminished in the mutant.
TS21: pituitary gland Present 2B -/- Expression in Rathke's pouch is unaffected in the mutant.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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04/23/2024
MGI 6.23 		The Jackson Laboratory