Gene Expression Data
Assay Details
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Assay
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Reference:
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J:175542
Anderson KR, et al., The L6 domain tetraspanin Tm4sf4 regulates endocrine pancreas differentiation and directed cell migration. Development. 2011 Aug;138(15):3213-24
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6433791
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Gene symbol:
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Tm4sf4
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Gene name:
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transmembrane 4 superfamily member 4
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Probe:
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Tm4sf4 cDNA2
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1A:
embryonic day 10.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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involves: Black Swiss
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: pancreas primordium |
Present |
Regionally restricted |
1A
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Expression in pancreatic epithelium as early as E10.5
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Specimen
1C:
(close )
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Genetic Background:
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involves: 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Nkx2-2tm1Jlr/Nkx2-2tm1Jlr
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1F:
embryonic day 12.5
(more )
Specimen
1F:
(close )
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Genetic Background:
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involves: Black Swiss
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1I:
(close )
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Genetic Background:
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involves: 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Nkx2-2tm1Jlr/Nkx2-2tm1Jlr
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1K:
embryonic day 15.5
(more )
Specimen
1K:
(close )
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Genetic Background:
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involves: Black Swiss
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1M:
embryonic day 15.5
(more )
Specimen
1M:
(close )
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Genetic Background:
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involves: Black Swiss
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1N:
embryonic day 15.5
(more )
Specimen
1N:
(close )
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Genetic Background:
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involves: Black Swiss
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: intestine |
Present |
Regionally restricted |
1N
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Expression in subset of intestinal enteroendocrine cells.
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Specimen
1O:
(close )
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Genetic Background:
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involves: 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Nkx2-2tm1Jlr/Nkx2-2tm1Jlr
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
1Q:
(close )
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Genetic Background:
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involves: 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Nkx2-2tm1Jlr/Nkx2-2tm1Jlr
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: liver |
Present |
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1Q
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Expression levels unchanged in mutant.
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Specimen
1R:
(close )
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Genetic Background:
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involves: 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Nkx2-2tm1Jlr/Nkx2-2tm1Jlr
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: intestine |
Present |
Regionally restricted |
1R
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Expression in intestine unaltered in mutant.
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Specimen
1S:
embryonic day 15.5
(more )
Note: Double labeled: blue - Tm4sf4 RNA; brown - Ins1 (assay MGI:6433799) and Ins2 (assay MGI:6433800).
Specimen
1S:
(close )
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Genetic Background:
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involves: Black Swiss
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Tm4sf4 RNA; brown - Ins1 (assay MGI:6433799) and Ins2 (assay MGI:6433800).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas epithelium |
Present |
Regionally restricted |
1S
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Co-expression with in only a rare number of insulin-expressing cells.
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Specimen
1T:
embryonic day 15.5
(more )
Note: Double labeled: blue - Tm4sf4 RNA; brown - Cpa1 (assay MGI:6433801).
Specimen
1T:
(close )
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Genetic Background:
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involves: Black Swiss
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Note:
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Double labeled: blue - Tm4sf4 RNA; brown - Cpa1 (assay MGI:6433801).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: pancreas epithelium |
Present |
Regionally restricted |
1T
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Higher expression in ductal truncal cells; lower expression in Cpa-expressing distal tip domains and exocrine tissue.
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Specimen
2C e15.5 Wildtype:
embryonic day 15.5
(more )
Specimen
2C e15.5 Wildtype:
(close )
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Genetic Background:
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involves: Black Swiss
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Specimen
2C e15.5 Ngn3-/-:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * Black Swiss
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Mutant Allele(s):
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Neurog3tm1(EGFP)Khk/Neurog3tm1(EGFP)Khk
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Cryosection
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Analysis Tools