Antibody:	Anti-AMHR2
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS28: cerebral cortex	Present		1a
TS28: dentate gyrus	Present		1a
TS28: hippocampus	Present		1a

Structure	Level	Pattern	Image	Note
TS28: brain	Absent		1b

Structure	Level	Pattern	Image	Note
TS28: hypothalamus	Present		1c
TS28: diencephalon	Present	Regionally restricted	1c	Expression was detected in the organum vasculosum of the lamina terminalis.

Structure	Level	Pattern	Image	Note
TS28: brain	Absent		1d

Structure	Level	Pattern	Image	Note
TS28: arcuate nucleus	Present		1e
TS28: dorsomedial hypothalamic nucleus	Present		1e
TS28: median eminence	Present		1e

Structure	Level	Pattern	Image	Note
TS28: arcuate nucleus	Present		1f
TS28: median eminence	Present		1f
TS28: hypothalamus	Present		1f	Expression was detected in hypothalamic tanycytes lining the third ventricle and endothelial cells.

Structure	Level	Pattern	Image	Note
TS28: brain	Absent		1g

Structure	Level	Pattern	Image	Note
TS20: nose	Present	Regionally restricted	2c , 2b	Expression of Amhr2 was detected in Gnrh1-positive neurons migrating out of the vomeronasal organ.

Structure	Level	Pattern	Image	Note
TS28: hypothalamus	Present	Regionally restricted	2f , 2i , 2e , 2h	More than 50% of Gnrh1-positive neurons were also immunopositive for Amhr2.

Structure	Level	Pattern	Image	Note
TS28: medial septal nucleus	Present	Regionally restricted	S1b , S1e , S1d	More than 50% of the Gnrh1-positive neurons were also immunopositive for Amhr2.

Structure	Level	Pattern	Image	Note
TS28: basal forebrain	Present	Regionally restricted	S1g , S1h	More than 50% of the Gnrh1-positive neurons were also immunopositive for Amhr2 in the diagonal band of Broca.