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Gene Expression Data
Assay Details
Assay
Reference: J:227045 Jaworski A, et al., Operational redundancy in axon guidance through the multifunctional receptor Robo3 and its ligand NELL2. Science. 2015 Nov 20;350(6263):961-5
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6430802
Gene symbol: Cntn2
Gene name: contactin 2
Antibody: anti-TAG-1 (4D7)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 4A/B: embryonic day 11.5 (more )
Note: Double labeled: red - Cntn2; green - Mnx1 (assay MGI:6430801).
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 4A , 4B Expressed in precrossing commissural axons. The majority of commissural axons avoided the region occupied by motor neurons.
Specimen 4C: embryonic day 11.5; Robo3tm1Matl/Robo3+ (more )
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 4C Expressed in precrossing commissural axons. The majority of commissural axons avoided the region occupied by motor neurons.
Specimen 4D: embryonic day 11.5; Robo3tm1Matl/Robo3tm1Matl (more )
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 4D Expressed in precrossing commissural axons. Many more (about sixfold greater) projected through the ventral-most portion of the motor column, compared with the controls.
Specimen 4E: embryonic day 11.5; Nell2tm1.1Matl/Nell2tm1.1Matl (more )
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 4E Expressed in precrossing commissural axons. The majority of commissural axons avoided the region occupied by motor neurons. No increase in axon growth through the motor column.
Specimen 4F: embryonic day 11.5; Nell2tm1.1Matl/Nell2tm1.1Matl, Robo3tm1Matl/Robo3+ (more )
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 4F Expressed in precrossing commissural axons. Significant increase (about threefold) in commissural axon growth through the motor column, compared with the controls.
Specimen 4G: embryonic day 11.5; Nell2tm1.1Matl/Nell2tm1.1Matl, Robo3tm1Matl/Robo3+ (more )
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 4G Expressed in precrossing commissural axons. Significant increase (about threefold) in commissural axon growth through the motor column, compared with the controls.
Specimen 4H: embryonic day 11.5; Nell2tm1.1Matl/Nell2tm1.1Matl, Robo3tm1Matl/Robo3+ (more )
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 4H Expressed in precrossing commissural axons. Significant increase (about threefold) in commissural axon growth through the motor column, compared with the controls.
Specimen 4I: embryonic day 11.5; Nell2tm1.1Matl/Nell2tm1.1Matl, Robo3tm1Matl/Robo3+ (more )
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted 4I Expressed in precrossing commissural axons. Significant increase (about threefold) in commissural axon growth through the motor column, compared with the controls.
Specimen S4E: embryonic day 11.5; Robo3tm1Matl/Robo3+ (more )
Note: Double labeled: red - Cntn2; green - Mnx1 (assay MGI:6430801).
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted S4E Expressed in precrossing commissural axons. The majority of commissural axons avoided the region occupied bymotor neurons.
Specimen S4F: embryonic day 11.5; Robo3tm1Matl/Robo3tm1Matl (more )
Note: Double labeled: red - Cntn2; green - Mnx1 (assay MGI:6430801).
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted S4F Expressed in precrossing commissural axons. Many more projected through the ventral-most portion of the motor column, compared with the controls.
Specimen S4G: embryonic day 11.5; Nell2tm1.1Matl/Nell2tm1.1Matl (more )
Note: Double labeled: red - Cntn2; green - Mnx1 (assay MGI:6430801).
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted S4G Expressed in precrossing commissural axons. The majority of commissural axons avoided the region occupied by motor neurons. No increase in axon growth through the motor column.
Specimen S4H: embryonic day 11.5; Nell2tm1.1Matl/Nell2tm1.1Matl, Robo3tm1Matl/Robo3+ (more )
Note: Double labeled: red - Cntn2; green - Mnx1 (assay MGI:6430801).
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted S4H Expressed in precrossing commissural axons. Significant increase (about threefold) in commissural axon growth through the motor column, compared with the controls.
Specimen S4I/J: embryonic day 11.5; Nell1l7R6-6R/Nell1l7R6-6R, Nell2tm1.1Matl/Nell2tm1.1Matl (more )
Note: Double labeled: red - Cntn2; green - Mnx1 (assay MGI:6430801).
Structure Level Pattern Image Note
TS19: future spinal cord Present Regionally restricted S4J , S4I Expressed in precrossing commissural axons. The majority of commissural axons avoided the region occupied by motor neurons. No increase in axon growth through the motor column.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/18/2025
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