Acta2 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:272568 Munoz-Martin N, et al., Myc is dispensable for cardiomyocyte development but rescues Mycn-deficient hearts through functional replacement and cell competition. Development. 2019 Feb 1;146(3):dev170753
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6403264
Gene symbol:	Acta2
Gene name:	actin alpha 2, smooth muscle, aorta

Antibody:	Anti-smooth muscle alpha-Actin (clone 1A4)
Detection system:	Secondary antibody coupled to Alexa Fluor 594

Results

2C left ©

2C middle ©

Specimen 2C: embryonic day 9.5; Myc^tm1Slek/Myc^tm1Slek (more

)
Note: Double labeled: red - Acta2; green - Myc (assay MGI:6403273).

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Present		2C middle , 2C left

4C left ©

Specimen 4C Wt: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present		4C left

4C right ©

Specimen 4C cKO-Mycn: embryonic day 10.5; Mycn^tm1Psk/Mycn^tm1Psk, Nkx2-5^tm2(cre)Rph/Nkx2-5⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Present		4C right

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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