Antibody:	anti-Oct-3/4 (H-134; sc-9081)
Detection system:	Secondary antibody coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS13: primordial germ cell of hindgut	Present	Single cells	3A	Expression is present in the primordial germ cells. 59% of those are located in the mesenchyme surrounding the gut and 39% are located in the gut epithelium.
TS13: primordial germ cell of trunk mesenchyme	Present	Single cells	3A	Expression is present in the primordial germ cells. 59% of those are located in the mesenchyme surrounding the gut and 39% are located in the gut epithelium.

Structure	Level	Pattern	Image	Note
TS15: primordial germ cell of gonad	Present	Single cells	3B	Expression is present in the primordial germ cells. 85% are migrating through the gut mesenchyme and mesentery, 7% are in the gut epithelium, and 8% have reached the location of the forming genital ridges.
TS15: primordial germ cell of hindgut	Present	Single cells	3B	Expression is present in the primordial germ cells. 85% are migrating through the gut mesenchyme and mesentery, 7% are in the gut epithelium, and 8% have reached the location of the forming genital ridges.
TS15: primordial germ cell of trunk mesenchyme	Present	Single cells	3B	Expression is present in the primordial germ cells. 85% are migrating through the gut mesenchyme and mesentery, 7% are in the gut epithelium, and 8% have reached the location of the forming genital ridges.

Structure	Level	Pattern	Image	Note
TS17: primordial germ cell of dorsal mesentery	Present	Single cells	3C	Expression is present in the primordial germ cells. 85% of them are located in the genital ridges, while 9% of them are in the gut mesenchyme, mesentery or the region surrounding the aorta.
TS17: primordial germ cell of gonad	Present	Single cells	3C	Expression is present in the primordial germ cells. 85% of them are located in the genital ridges, while 9% of them are in the gut mesenchyme, mesentery or the region surrounding the aorta.
TS17: primordial germ cell of trunk mesenchyme	Present	Single cells	3C	Expression is present in the primordial germ cells. 85% of them are located in the genital ridges, while 9% of them are in the gut mesenchyme, mesentery or the region surrounding the aorta.

Structure	Level	Pattern	Image	Note
TS19: primordial germ cell	Present	Single cells	3D	Expression is present in the primordial germ cells. >90% of them are located in the gonads; most of the remaining cells are located in the peri-aortic region or the mesentery, where they are often found in clusters.
TS19: primordial germ cell of gonad	Present	Single cells	3D	Expression is present in the primordial germ cells. >90% of them are located in the gonads; most of the remaining cells are located in the peri-aortic region or the mesentery, where they are often found in clusters.

Structure	Level	Pattern	Image	Note
TS19: primordial germ cell	Present	Single cells	4A	Expression is present in the primordial germ cells. A minority of them (<10%) are located in the peri-aortic region or the mesentery.

Structure	Level	Pattern	Image	Note
TS19: primordial germ cell	Present	Single cells	4B	Expression is present in the primordial germ cells. >90% of them are located in the gonads; most of the remaining cells are located in the peri-aortic region or the mesentery.
TS19: primordial germ cell of gonad	Present	Single cells	4B	Expression is present in the primordial germ cells. >90% of them are located in the gonads; most of the remaining cells are located in the peri-aortic region or the mesentery.

Structure	Level	Pattern	Image	Note
TS17: primordial germ cell of gonad	Present	Single cells	5A , 5B