Gene Expression Data
Assay Details
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Assay
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Reference:
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J:242062
Wolfe AD, et al., STELLA collaborates in distinct mesendodermal cell subpopulations at the fetal-placental interface in the mouse gastrula. Dev Biol. 2017 May 01;425(1):44-57
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6401472
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Gene symbol:
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Pou5f1
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Gene name:
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POU domain, class 5, transcription factor 1
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Results
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Specimen
2A/D:
embryonic day 7.5
(more  )
Note: Late headfold stage. Triple labeled: red - Oct-3/4 (Pou5f1); green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
Specimen
2A/D:
(close  )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Late headfold stage. Triple labeled: red - Oct-3/4 (Pou5f1); green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: allantois mesoderm |
Present |
Regionally restricted |
2A
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2D
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Expression in Allantoic Core Domain (ACD) with some cells co-expressing Stella (Dppa3) and others lacking Stella expression.
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Specimen
2E/H:
embryonic day 8.0
(more )
Note: 6 somite stage. Triple labeled: red - Oct-3/4 (Pou5f1); green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
Specimen
2E/H:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somite stage. Triple labeled: red - Oct-3/4 (Pou5f1); green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: allantois mesenchyme |
Present |
Regionally restricted |
2E
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2H
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Expression in remnant of Allantoic Core Domain (ACD) distal to invaginating hindgut with all expressing cells co-expressing Stella (Dppa3). Expression more concentrated in core of nucleus.
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Specimen
2I/L:
embryonic day 8.5
(more )
Note: 9 somite stage. Triple labeled: red - Oct-3/4 (Pou5f1); green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
Specimen
2I/L:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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9 somite stage. Triple labeled: red - Oct-3/4 (Pou5f1); green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: allantois mesenchyme |
Present |
Regionally restricted |
2I
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2L
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Co-expression with Stella (Dppa3) in cell cluster at midline base of allantois, with occasional expressing cells moving distal from cluster.
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Specimen
3A/D:
embryonic day 7.5
(more )
Note: Late headfold stage. Triple labeled: red - Oct-3/4 (Pou5f1); green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
Specimen
3A/D:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Late headfold stage. Triple labeled: red - Oct-3/4 (Pou5f1); green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: posterior visceral endoderm |
Present |
Regionally restricted |
3D
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3A
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In allantois-associated posterior visceral endoderm (aPVE), co-expression with Stella (Dppa3) in some cells without Stella.
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Specimen
2s stage not shown:
embryonic day 8.0
(more )
Note: 2 somite stage.
Specimen
2s stage not shown:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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2 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: posterior visceral endoderm |
Present |
Regionally restricted |
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Nearly all expressing cels in allantois-associated posterior visceral endoderm (aPVE) co-express Stella (Dppa3).
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Specimen
3E/H:
embryonic day 8.0
(more )
Note: 6 somite stage. Triple labeled: red - Oct-3/4 (Pou5f1); green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
Specimen
3E/H:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somite stage. Triple labeled: red - Oct-3/4 (Pou5f1); green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: primitive hindgut |
Present |
Regionally restricted |
3E
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3H
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Co-expression with Stella (Dppa3) in all expressing cells in visceral endoderm of hindgut lip. Expression more concentrated in core of nucleus.
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Specimen
3I/L:
embryonic day 8.0
(more )
Note: 6 somite stage. Triple labeled: red - Oct-3/4 (Pou5f1); green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
Specimen
3I/L:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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6 somite stage. Triple labeled: red - Oct-3/4 (Pou5f1); green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: hindgut diverticulum endoderm |
Present |
Regionally restricted |
3I
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3L
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Co-expression with Stella (Dppa3) in all expressing cells in ventral hindgut endoderm. Expression more concentrated in core of nucleus.
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Specimen
3O/R:
embryonic day 8.5
(more )
Note: 9 somite stage. Triple labeled: red - Oct-3/4 (Pou5f1); green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
Specimen
3O/R:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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9 somite stage. Triple labeled: red - Oct-3/4 (Pou5f1); green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: primitive hindgut |
Present |
Regionally restricted |
3O
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3R
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Expression in increased numbers of cells co-expressing with Stella (Dppa3) throughout hindgut lip. Co-expressing cells remain clusteerd within hindgut lip, not in any other visceral endoderm.
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Specimen
3S/V:
embryonic day 8.5
(more )
Note: 9 somite stage. Triple labeled: red - Oct-3/4 (Pou5f1); green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
Specimen
3S/V:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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9 somite stage. Triple labeled: red - Oct-3/4 (Pou5f1); green - Stella (Dppa3 assay MGI:6401470); blue - DAPI.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS13: hindgut diverticulum |
Present |
Widespread |
3S
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3V
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Increased numbers of cells co-expressing with Stella (Dppa3) throughout ventral hindgut, spanning longitudinal extent of hindgut. Expression more concentrated in core of nucleus.
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Specimen
LHF stage not shown:
embryonic day 7.5
(more )
Note: Late headfold stage.
Specimen
LHF stage not shown:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 7.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Late headfold stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS11: allantois mesoderm |
Present |
Single cells |
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Rare Allantoic Core Domain cells co-expressed Oct-3/4 (Pou5f1), Stella (Dppa3) and T, but only at the LHF stage.
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Specimen
12s stage not shown:
embryonic day 8.5
(more )
Note: 12 somite stage.
Specimen
12s stage not shown:
(close )
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Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 8.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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12 somite stage.
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| Structure |
Level |
Pattern |
Image |
Note |
| TS12: foregut diverticulum |
Absent |
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Co-expressng cells not observed within foregut.
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| TS12: posterior visceral endoderm |
Present |
Regionally restricted |
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Co-expressng cells not observed in posterior visceral endoderm anterior to the site of hingut invagination, through the 12s stage.
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Analysis Tools