Tubb3 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:285046 Law S, et al., Expression of MYOSIN VIIA in developing mouse cochleovestibular ganglion neurons. Gene Expr Patterns. 2020 Jan 7;35:119092
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6393617
Gene symbol:	Tubb3
Gene name:	tubulin, beta 3 class III

Antibody:	anti-Tubulin beta 3 (clone TUJ1, MMS-435P/801202)
Detection system:	Alexa Fluor 488

Results

1D ©

1F ©

Specimen 1D/F: embryonic day 9.5 (more

)
Note: Double labeled: green - Tubb3; red - Myo7a (assay MGI:6393614).

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present	Regionally restricted	1D , 1F	Cytoplasmic expression in cochleovestibular ganglion and geniculate ganglion.

1F inset ©

Specimen 1F inset: embryonic day 9.5 (more

)
Note: Double labeled: green - Tubb3; red - Myo7a (assay MGI:6393614).

Structure	Level	Pattern	Image	Note
TS15: facio-acoustic preganglion complex	Present	Regionally restricted	1F inset	Co-expression with Myo7a in cochleovestibular ganglion.

2D ©

2F ©

Specimen 2D/F: embryonic day 10.5 (more

)
Note: Double labeled: green - Tubb3; red - Myo7a (assay MGI:6393614).

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present	Regionally restricted	2D , 2F	Cytoplasmic expression in neurons in cochleovestibular ganglion and geniculate ganglion.
TS17: facial ganglion	Present	Regionally restricted	2D , 2F	Cytoplasmic expression in neurons in cochleovestibular ganglion and geniculate ganglion.

2F inset ©

Specimen 2F inset: embryonic day 10.5 (more

)
Note: Double labeled: green - Tubb3; red - Myo7a (assay MGI:6393614).

Structure	Level	Pattern	Image	Note
TS17: acoustic ganglion	Present	Regionally restricted	2F inset	Co-expression with Myo7a in cochleovestibular ganglion.

2J ©

2L ©

Specimen 2J/L: embryonic day 11.5 (more

)
Note: Double labeled: green - Tubb3; red - Myo7a (assay MGI:6393614).

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Present	Regionally restricted	2J , 2L	Cytoplasmic expression in all neurons in cochleovestibular ganglion and geniculate ganglion.
TS19: facial ganglion	Present	Regionally restricted	2J , 2L	Cytoplasmic expression in all neurons in cochleovestibular ganglion and geniculate ganglion.

2L inset ©

Specimen 2L inset: embryonic day 11.5 (more

)
Note: Double labeled: green - Tubb3; red - Myo7a (assay MGI:6393614).

Structure	Level	Pattern	Image	Note
TS19: acoustic ganglion	Present	Regionally restricted	2L inset	Co-expression with Myo7a in cochleovestibular ganglion.

3A ©

3C ©

Specimen 3A/C: embryonic day 12.5 (more

)
Note: Double labeled: green - Tubb3; red - Myo7a (assay MGI:6393614).

Structure	Level	Pattern	Image	Note
TS20: cochlear ganglion	Present	Regionally restricted	3C , 3A	Cytoplasmic expression in neurons in vestibular ganglion and cochlear ganglion.
TS20: vestibular ganglion	Present	Regionally restricted	3C , 3A	Cytoplasmic expression in neurons in vestibular ganglion and cochlear ganglion.
TS20: vestibular component epithelium	Absent		3C , 3A	No expression in presumptive vestibular hair cells in otic epithelium.

3C inset ©

Specimen 3C inset: embryonic day 12.5 (more

)
Note: Double labeled: green - Tubb3; red - Myo7a (assay MGI:6393614).

Structure	Level	Pattern	Image	Note
TS20: vestibular ganglion	Present	Regionally restricted	3C inset	Co-expression with Myo7a in vestibular ganglion.

3D ©

3F ©

Specimen 3D/F: embryonic day 13.5 (more

)
Note: Double labeled: green - Tubb3; red - Myo7a (assay MGI:6393614).

Structure	Level	Pattern	Image	Note
TS21: cochlear ganglion	Present	Regionally restricted	3D , 3F	Cytoplasmic expression in nerons in vestibular ganglion, cochlear ganglion.
TS21: vestibular ganglion	Present	Regionally restricted	3D , 3F	Cytoplasmic expression in nerons in vestibular ganglion, cochlear ganglion.
TS21: vestibular component epithelium	Present	Regionally restricted	3F , 3D	Expression in neuronal projection in otic epithelium. No expression in vestibular hair cells.

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