Antibody:	anti-Phox2a
Detection system:	Biotinylated secondary antibody/[Strept]avidin coupled to horseradish peroxidase

Structure	Level	Pattern	Image	Note
TS17: facial ganglion	Present		1B
TS17: glossopharyngeal ganglion	Present		1B
TS17: vagus ganglion	Present		1B
TS17: nodose placode	Present		1B	Expression includes the delaminating neuroblasts.
TS17: petrosal placode	Present		1B	Expression includes the delaminating neuroblasts.

Structure	Level	Pattern	Image	Note
TS17: sympathetic nervous system	Present	Regionally restricted	2A	Expression is in the primordia of the sympathetic chain on both sides of the dorsal aorta.

Structure	Level	Pattern	Image	Note
TS21: superior cervical ganglion	Strong		2B
TS21: glossopharyngeal ganglion	Weak	Regionally restricted	2B	Expression is distal.
TS21: vagus ganglion	Weak	Regionally restricted	2B	Expression is distal.

Structure	Level	Pattern	Image	Note
TS21: sphenopalatine ganglion	Present		3A
TS21: trigeminal ganglion	Absent		3A

Structure	Level	Pattern	Image	Note
TS21: submandibular ganglion	Present		3B

Structure	Level	Pattern	Image	Note
TS21: enteric nervous system	Present		3C	Expression is in the myenteric plexus.

Structure	Level	Pattern	Image	Note
TS19: neural tube	Present	Regionally restricted	4A	Expression is in the lateral neural tube, just dorsal to the motoneuron columns throughout cervical, thoracic, and lumbar levels.

Structure	Level	Pattern	Image	Note
TS19: hindbrain	Present	Regionally restricted	4B	Expression is in a broad region in the dorsal half of the basal plate, which in some areas appeared subdivided into a superficial and a deep layer.

Structure	Level	Pattern	Image	Note
TS19: metencephalon basal plate mantle layer	Present		4C
TS19: hindbrain lateral wall	Present	Single cells	4C	Expression is in a few postmitotic neurons migrating toward the mantle layer.
TS19: hindbrain floor plate	Absent		4C

Structure	Level	Pattern	Image	Note
TS19: myelencephalon lateral wall	Present	Regionally restricted	4D	Expression is in myelencephalon with a rostral limit of expression at the fourth-third inter-rhombomeric boundary and expression continues caudally into the fifth rhombomere.

Structure	Level	Pattern	Image	Note
TS19: hindbrain lateral wall	Present	Regionally restricted	5A	At the level of the first rhombomere expression is in a thin column.
TS19: midbrain-hindbrain junction	Present		5A

Structure	Level	Pattern	Image	Note
TS19: midbrain-hindbrain junction	Present		5B	Expression is in two streams of cells originating just caudally and just rostrally to the fovea isthmi.

Structure	Level	Pattern	Image	Note
TS21: pons	Present	Regionally restricted	5C	Expression is in the locus coeruleus with more caudally located scattered cells of the subcoeruleus.

Structure	Level	Pattern	Image	Note
TS21: pons	Present	Regionally restricted	5D	Expression is in the locus coeruleus with more caudally located scattered cells of the subcoeruleus.

Structure	Level	Pattern	Image	Note
TS28: locus coeruleus	Present		6A
TS28: vestibular nucleus	Present		6A

Structure	Level	Pattern	Image	Note
TS28: locus coeruleus	Present		6B
TS28: vestibular nucleus	Present		6B

Structure	Level	Pattern	Image	Note
TS28: lateral medullary reticular formation	Present	Regionally restricted	6C	Expression is in the noradrenergic nucleus A1 of the ventrolateral medulla.

Structure	Level	Pattern	Image	Note
TS28: nucleus ambiguus	Present	Regionally restricted	6D	Expression is trace or absent at the more caudal and rostral level.

Structure	Level	Pattern	Image	Note
TS28: dorsal motor nucleus of vagus nerve	Present		6E
TS28: solitary tract nucleus	Present		6E
TS28: hypoglossal nucleus	Absent		6E

Structure	Level	Pattern	Image	Note
TS28: facial nucleus	Present	Regionally restricted	6F	Expression is in a fraction of the facial motoneurons.

Structure	Level	Pattern	Image	Note
TS17: enteric nervous system	Present	Regionally restricted		Expression is in the myenteric plexus.

Structure	Level	Pattern	Image	Note
TS19: facial ganglion	Strong	Regionally restricted		Expression is distal.
TS19: glossopharyngeal ganglion	Strong	Regionally restricted		Expression is distal.
TS19: vagus ganglion	Strong	Regionally restricted		Expression is distal.

Structure	Level	Pattern	Image	Note
TS21: cervico-thoracic ganglion	Strong
TS21: pelvic ganglion	Strong
TS21: adrenal gland	Strong	Regionally restricted		Expression is in the adrenal medulla.
TS21: sympathetic ganglion	Strong	Regionally restricted		Expression includes the prevertebral ganglion.
TS21: otic ganglion	Present
TS21: paracardiac ganglion	Present
TS21: medulla oblongata	Present	Regionally restricted		Expression is in the lateral tegmentum of the medulla.
TS21: dorsal root ganglion	Absent
TS21: vestibulo-cochlear ganglion	Absent

Structure	Level	Pattern	Image	Note
TS24: myenteric nerve plexus	Present