Gene Expression Data
Assay Details
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Assay
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Reference:
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J:270238
Wang L, et al., Dynamic expression of p75(NTR) and Lingo-1 during development of mouse retinofugal pathway. Neurosci Lett. 2018 Sep 7;686:106-111
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6386237
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Gene symbol:
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Ngfr
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Gene name:
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nerve growth factor receptor (TNFR superfamily, member 16)
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Antibody:
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Anti-p75NTR
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Detection system:
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Secondary antibody coupled to Alexa Fluor
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Results
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Specimen
1A:
embryonic day 13.5
(more  )
Specimen
1A:
(close  )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: retina |
Present |
Regionally restricted |
1A
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Expression was detected in neuroepithelial cells that appear to migrate from the basal layer to vitreal regions of the retina. Expression was also observed in the optic fiber layer.
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Specimen
1B:
embryonic day 14.5
(more )
Specimen
1B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: retina |
Present |
Regionally restricted |
1B
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Expression was detected in the ganglion cell layer and the optic fiber layer.
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Specimen
1C:
embryonic day 15.5
(more )
Specimen
1C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: retina |
Present |
Regionally restricted |
1C
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The immunostaining pattern was maintained at E15.5, showing a preferential localization on optic axons.
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Specimen
2A:
embryonic day 13.5
(more )
Specimen
2A:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: optic disc |
Present |
Regionally restricted |
2A
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At the optic disc, immunoreactivity was found on the optic axons. Only a weak signal was detected in the radial glial profiles in the region.
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Specimen
2B:
embryonic day 14.5
(more )
Specimen
2B:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: optic disc |
Present |
Regionally restricted |
2B
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At the optic disc, immunoreactivity was found on the optic axons. Only a weak signal was detected in the radial glial profiles in the region.
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Specimen
2C:
embryonic day 15.5
(more )
Specimen
2C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: optic disc |
Present |
Regionally restricted |
2C
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At the optic disc, immunoreactivity was found on the optic axons. Only a weak signal was detected in the radial glial profiles in the region.
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Specimen
3A:
embryonic day 13.5
(more )
Specimen
3A:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 13.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS21: optic stalk |
Present |
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3A
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3B
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Expression was detected on the axons in the optic stalk.
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Specimen
3C:
embryonic day 14.5
(more )
Specimen
3C:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Specimen
3D:
embryonic day 15.5
(more )
Specimen
3D:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Specimen
3E:
embryonic day 15.5
(more )
Specimen
3E:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 15.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS23: optic chiasma |
Present |
Regionally restricted |
3E
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Expression was found in the optic axon layer of the chiasm.
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Specimen
3G:
embryonic day 14.5
(more )
Note: Double labeled: red - Ngfr; green - Nes (assay MGI:6386239).
Specimen
3G:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Double labeled: red - Ngfr; green - Nes (assay MGI:6386239).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: optic chiasma |
Present |
Regionally restricted |
3I
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3G
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Expression in the optic chiasm was not co-localized with radial glial cell marker RC2 (Nestin).
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Specimen
3J:
embryonic day 14.5
(more )
Note: Double labeled: red - Ngfr; green - Fut4 (assay MGI:6386240).
Specimen
3J:
(close )
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Genetic Background:
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C57BL/6J
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Age:
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embryonic day 14.5
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Age Note:
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Age normalized so that noon of plug day = E0.5.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Double labeled: red - Ngfr; green - Fut4 (assay MGI:6386240).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: optic chiasma |
Present |
Regionally restricted |
3L
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3J
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Expression was not co-localized with chiasmatic neuron marker SSEA-1 (Fut4).
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Analysis Tools