Gene Expression Data
Assay Details
Assay
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Reference:
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J:135657
Hayashi T, et al., Hesr1 and Hesr2 may act as early effectors of Notch signaling in the developing cochlea. Dev Biol. 2008 Apr 1;316(1):87-99
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6385192
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Gene symbol:
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Hey2
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Gene name:
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hairy/enhancer-of-split related with YRPW motif 2
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Probe:
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Hesr2 probe1
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Results
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Specimen
1B:
embryonic day 12.5
(more )
Specimen
1B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS20: cochlear duct |
Present |
Regionally restricted |
1B
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Narrow band of expression, nested in Hesr1 expression region on ventral side of cochlear duct.
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Specimen
1E:
embryonic day 13.5
(more )
Specimen
1E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cochlear duct |
Present |
Regionally restricted |
1E
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Narrow band of expression, nested in Hesr1 expression region on ventral side of cochlear duct.
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Specimen
1G:
embryonic day 14.5
(more )
Specimen
1G:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS22: cochlear duct |
Strong |
Regionally restricted |
1G
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Expression more intense at all turns of cochlea, overlapping almost entirely with Hesr1 expression domain.
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Specimen
1I:
embryonic day 16.5
(more )
Specimen
1I:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS24: cochlear duct |
Weak |
Regionally restricted |
1I
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Expression downregulated but continues in apex. No expression in basal turns (1 and 2).
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Specimen
1K:
embryonic day 18.5
(more )
Specimen
1K:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 18.5
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Sex:
|
Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS26: cochlear duct |
Weak |
Regionally restricted |
1K
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Expression dowregulated but continues at apex. No expression in turns 1,2 and 3.
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Specimen
P0 ont shown:
postnatal day 0
(more )
Specimen
P0 ont shown:
(close )
Genetic Background:
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Not Specified
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Specimen
S1B:
embryonic day 12.5
(more )
Specimen
S1B:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Specimen
S1E:
embryonic day 15.5
(more )
Specimen
S1E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Specimen
S1I:
embryonic day 15.5
(more )
Specimen
S1I:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: saccule |
Absent |
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S1I
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No expression in vestibular system at any age examined.
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Specimen
3A:
embryonic day 15.5
(more )
Specimen
3A:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Structure |
Level |
Pattern |
Image |
Note |
TS23: cochlear duct |
Present |
Regionally restricted |
3A
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Expression throughout cochlea (turns 1 to 4).
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Specimen
4E:
embryonic day 13.5
(more )
Note: Double labeled: purple - Hesr2 (Hey2); red - p27kip1 (Cdkn1b assay MGI:6385184).
Specimen
4E:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled: purple - Hesr2 (Hey2); red - p27kip1 (Cdkn1b assay MGI:6385184).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: cochlear duct |
Present |
Regionally restricted |
4E
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Expression in middle part of cochlear duct. Expression domain included p27kip1 (Cdkn1b) domain but extends more mdeially.
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Specimen
4F:
embryonic day 15.5
(more )
Note: Double labeled: purple - Hesr2 (Hey2); red - p27kip1 (Cdkn1b assay MGI:6385184).
Specimen
4F:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Carnoy's Fixative
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Embedding:
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Paraffin
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Note:
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Double labeled: purple - Hesr2 (Hey2); red - p27kip1 (Cdkn1b assay MGI:6385184).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: cochlear duct |
Present |
Regionally restricted |
4F
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Expression in basal turn (turn 1). Expression more restricted than that of Hesr1 and is completely contained withn p27kip1 (Cdkn1b) domain.
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