Antibody:	PITX2B antibody
Detection system:	Secondary antibody coupled to Cy3

Structure	Level	Pattern	Image	Note
TS15: heart	Strong		2B , 2C	About 50% of the nuclei stained positive for Pitx2b.

Structure	Level	Pattern	Image	Note
TS19: heart	Strong		2E , 2D	About 40% of the nuclei stained positive for Pitx2b.

Structure	Level	Pattern	Image	Note
TS21: heart	Present		2G , 2F	About 20% of the nuclei stained positive for Pitx2b.

Structure	Level	Pattern	Image	Note
TS23: heart	Weak		2I , 2H	About 10% of the nuclei stained positive for Pitx2b.

Structure	Level	Pattern	Image	Note
TS25: heart	Weak		2J , 2K	About 6-7% of the nuclei stained positive for Pitx2b.

Structure	Level	Pattern	Image	Note
TS27: heart	Weak		2M , 2L	About 6-7% of the nuclei stained positive for Pitx2b.

Structure	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	Present		3B , 3C , 3A
TS19: endocardial lining	Present		3B , 3C , 3A
TS19: visceral pericardium	Present		3B , 3C , 3A

Structure	Level	Pattern	Image	Note
TS21: heart right ventricle	Present		4B , 4C
TS21: interventricular septum	Present		4D , 4B
TS21: heart left ventricle	Present		4E , 4B

Structure	Level	Pattern	Image	Note
TS23: right atrium	Present		4F , 4G
TS23: interventricular septum	Present		4H , 4F
TS23: heart left ventricle	Present		4I , 4F

Structure	Level	Pattern	Image	Note
TS15: atrioventricular canal	Weak		5E , 5F	About 4% of the nuclei stained positive for Pitx2b.

Structure	Level	Pattern	Image	Note
TS19: atrioventricular canal	Weak		5H , 5G	About 4% of the nuclei stained positive for Pitx2b.

Structure	Level	Pattern	Image	Note
TS21: tricuspid valve	Present		5I , 5J	About 23% of the nuclei in the mural portion stained positive for Pitx2b whereas the septal portion had only 3% positive nuclei.
TS21: mitral valve	Weak		5I , 5K	About 3-7% of the nuclei stained positive for Pitx2b.

Structure	Level	Pattern	Image	Note
TS15: cardiac muscle tissue	Strong
TS15: heart atrium	Strong
TS15: primitive ventricle	Strong
TS15: atrioventricular canal	Weak			About 4% of the nuclei stained positive for Pitx2b.

Structure	Level	Pattern	Image	Note
TS17: cardiac muscle tissue	Strong
TS17: endocardial lining	Strong
TS17: visceral pericardium	Strong

Structure	Level	Pattern	Image	Note
TS19: cardiac muscle tissue	Strong
TS19: endocardial lining	Strong
TS19: heart left ventricle	Strong
TS19: heart right ventricle	Strong
TS19: interventricular septum	Strong
TS19: left atrium	Strong
TS19: right atrium	Strong
TS19: visceral pericardium	Strong
TS19: atrioventricular canal	Weak			About 4% of the nuclei stained positive for Pitx2b.

Structure	Level	Pattern	Image	Note
TS21: cardiac muscle tissue	Present
TS21: endocardial lining	Present
TS21: visceral pericardium	Present
TS21: heart left ventricle	Present			About 14-16% of the cells stained positive for Pix2b.
TS21: heart right ventricle	Present			About 14-16% of the cells stained positive for Pix2b.
TS21: interventricular septum	Present			About 28-31% of the cells stained positive for Pix2b.
TS21: left atrium	Present			About 28-31% of the cells stained positive for Pix2b.
TS21: right atrium	Present			About 28-31% of the cells stained positive for Pix2b.
TS21: tricuspid valve	Present	Regionally restricted		Expression is in the mural portion and is weak in the septal portion.
TS21: mitral valve	Weak			About 3-7% of the nuclei stained positive for Pitx2b.

Structure	Level	Pattern	Image	Note
TS23: cardiac muscle tissue	Weak
TS23: endocardial lining	Weak
TS23: visceral pericardium	Weak
TS23: interventricular septum	Weak			About 4% of the cells stained positive for Pix2b.
TS23: left atrium	Weak			About 10% of the cells stained positive for Pix2b.
TS23: right atrium	Weak			About 10% of the cells stained positive for Pix2b.
TS23: heart left ventricle	Weak			About 11-12% of the cells stained positive for Pix2b.
TS23: heart right ventricle	Weak			About 11-12% of the cells stained positive for Pix2b.

Structure	Level	Pattern	Image	Note
TS25: cardiac muscle tissue	Weak			About 5-6% of the nuclei stained positive for Pitx2b.
TS25: endocardial lining	Weak			About 5-6% of the nuclei stained positive for Pitx2b.
TS25: heart left ventricle	Weak			About 5-6% of the nuclei stained positive for Pitx2b.
TS25: heart right ventricle	Weak			About 5-6% of the nuclei stained positive for Pitx2b.
TS25: interventricular septum	Weak			About 5-6% of the nuclei stained positive for Pitx2b.
TS25: left atrium	Weak			About 5-6% of the nuclei stained positive for Pitx2b.
TS25: right atrium	Weak			About 5-6% of the nuclei stained positive for Pitx2b.
TS25: visceral pericardium	Weak			About 5-6% of the nuclei stained positive for Pitx2b.

Structure	Level	Pattern	Image	Note
TS27: cardiac muscle tissue	Weak			About 5-6% of the nuclei stained positive for Pitx2b.
TS27: endocardial lining	Weak			About 5-6% of the nuclei stained positive for Pitx2b.
TS27: heart left ventricle	Weak			About 5-6% of the nuclei stained positive for Pitx2b.
TS27: heart right ventricle	Weak			About 5-6% of the nuclei stained positive for Pitx2b.
TS27: interventricular septum	Weak			About 5-6% of the nuclei stained positive for Pitx2b.
TS27: left atrium	Weak			About 5-6% of the nuclei stained positive for Pitx2b.
TS27: right atrium	Weak			About 5-6% of the nuclei stained positive for Pitx2b.
TS27: visceral pericardium	Weak			About 5-6% of the nuclei stained positive for Pitx2b.