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Gene Expression Data
Assay Details
Assay
Reference: J:130196 Alfonsi F, et al., LIFR beta plays a major role in neuronal identity determination and glial differentiation in the mouse facial nucleus. Dev Biol. 2008 Jan 1;313(1):267-78
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6382047
Gene symbol: Phox2b
Gene name: paired-like homeobox 2b
Antibody: anti-Phox2b
Results
Specimen 3A: embryonic day 11.5 (more )
Note: Double labeled: green - Phox2b protein; red - Nkx2-2 RNA (assay MGI:6382030).
Structure Level Pattern Image Note
TS19: hindbrain Present 3A
Specimen 3B: embryonic day 11.5; Lifrtm1Imx/Lifrtm1Imx (more )
Note: Double labeled: green - Phox2b protein; red - Nkx2-2 RNA (assay MGI:6382030).
Structure Level Pattern Image Note
TS19: hindbrain Present 3B Authors report similar pattern of expression in rhombomere 4 in wild type and mutant embryos.
Specimen E12.5 +/+ not shown: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: hindbrain Present
Specimen E12.5 Lifr-/- not shown: embryonic day 12.5; Lifrtm1Imx/Lifrtm1Imx (more )
Structure Level Pattern Image Note
TS20: hindbrain Present Authors report similar pattern of expression in rhombomere 4 in wild type and mutant embryos.
Specimen S1I: embryonic day 12.5 (more )
Structure Level Pattern Image Note
TS20: brainstem nucleus Present S1I
Specimen S1J: embryonic day 12.5; Lifrtm1Imx/Lifrtm1Imx (more )
Structure Level Pattern Image Note
TS20: brainstem nucleus Present S1J No significant difference in expression between control and mutant embryos.
Specimen S1K: embryonic day 14.5 (more )
Structure Level Pattern Image Note
TS22: facial nucleus Present S1K
Specimen S1L: embryonic day 14.5; Lifrtm1Imx/Lifrtm1Imx (more )
Structure Level Pattern Image Note
TS22: facial nucleus Weak S1L Decrease in expression compared to control embryos.
Specimen S1M: embryonic day 16.5 (more )
Structure Level Pattern Image Note
TS24: facial nucleus Present S1M
Specimen S1N: embryonic day 16.5; Lifrtm1Imx/Lifrtm1Imx (more )
Structure Level Pattern Image Note
TS24: facial nucleus Weak S1N Decrease in expression compared to control embryos.
Specimen 4J: embryonic day 14.5; Lifrtm1Imx/Lifr+ (more )
Note: Double labeled: green - Phox2b; red - Lifr reporter (assay MGI:6382065).
Structure Level Pattern Image Note
TS22: facial nucleus Present 4J
Specimen 4K: embryonic day 14.5; Lifrtm1Imx/Lifr+ (more )
Note: Double labeled: green - Phox2b; red - Lifr reporter (assay MGI:6382065).
Structure Level Pattern Image Note
TS22: facial nucleus Present Regionally restricted 4K Expression in Lifrb-expressing cells.
Specimen 4L: embryonic day 14.5; Lifrtm1Imx/Lifrtm1Imx (more )
Note: Double labeled: green - Phox2b; red - Lifr reporter (assay MGI:6382065).
Structure Level Pattern Image Note
TS22: facial nucleus Weak 4L Authors report partial loss of expression compared to control.
Specimen 4M: embryonic day 14.5; Lifrtm1Imx/Lifrtm1Imx (more )
Note: Double labeled: green - Phox2b; red - Lifr reporter (assay MGI:6382065).
Structure Level Pattern Image Note
TS22: facial nucleus Weak Regionally restricted 4M Partial loss of expression in Lifbr-expressing cells compared to control.
Specimen 4R: embryonic day 16.5 (more )
Note: Double labeled: green - Phox2b protein; red - Isl1 protein (assay MGI:6382041).
Structure Level Pattern Image Note
TS24: facial nucleus Present 4R
Specimen 4S: embryonic day 16.5 (more )
Note: Double labeled: green - Phox2b protein; red - Isl1 protein (assay MGI:6382041).
Structure Level Pattern Image Note
TS24: facial nucleus Present Regionally restricted 4S Expression in Isl1-expressing cells.
Specimen 4T: embryonic day 16.5; Lifrtm1Imx/Lifrtm1Imx (more )
Note: Double labeled: green - Phox2b protein; red - Isl1 protein (assay MGI:6382041).
Structure Level Pattern Image Note
TS24: facial nucleus Weak 4T Partial loss of expression compared to control.
Specimen 4U: embryonic day 16.5; Lifrtm1Imx/Lifrtm1Imx (more )
Note: Double labeled: green - Phox2b protein; red - Isl1 protein (assay MGI:6382041).
Structure Level Pattern Image Note
TS24: facial nucleus Weak Regionally restricted 4U Partial loss of expression in Isl2-expressing cells compared to control.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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