Wnt3a RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:122556 Inoue T, et al., Zic2 and Zic3 synergistically control neurulation and segmentation of paraxial mesoderm in mouse embryo. Dev Biol. 2007 Jun 15;306(2):669-84
Assay type:	RNA in situ
MGI Accession ID:	MGI:6377223
Gene symbol:	Wnt3a
Gene name:	wingless-type MMTV integration site family, member 3A

Probe:	Wnt3a probe2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Results

9D ©

Specimen 9D: embryonic day 8.5 (more

)
Note: Also stained for Otx2 (assay MGI:6377219).

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present		9D

9E ©

Specimen 9E: embryonic day 8.5; Zic3^Bn/Y (more

)
Note: Also stained for Otx2 (assay MGI:6377219).

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Present		9E

9F (left) ©

Specimen 9F (left): embryonic day 8.5; Zic2^tm1Jaru/Zic2^tm1Jaru, Zic3^Bn/Y (more

)
Note: Also stained for Otx2 (assay MGI:6377219).

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Weak	Regionally restricted	9F (left)	Expression generally reduced in size, and in intensity in 43% of kd/+ Bn/Y mutants.

9F (right) ©

Specimen 9F (right): embryonic day 8.5; Zic2^tm1Jaru/Zic2^tm1Jaru, Zic3^Bn/Y (more

)
Note: Also stained for Otx2 (assay MGI:6377219).

Structure	Level	Pattern	Image	Note
TS13: primitive streak	Weak	Regionally restricted	9F (right)	Expression generally reduced in size, and in intensity in 43% of kd/+ Bn/Y mutants.

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24