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Gene Expression Data
Assay Details
Assay
Reference: J:279271 Ellis K, et al., GSK3 regulates hair cell fate in the developing mammalian cochlea. Dev Biol. 2019 Sep 15;453(2):191-205
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6364592
Gene symbol: Gsk3b
Gene name: glycogen synthase kinase 3 beta
Antibody: anti-GSK3beta (12456)
Detection system: Alexa Fluor 647
Results
Specimen 2A'/2A": embryonic day 14.5 (more )
Note: Double labeled: pink - Gsk3b; green - F-actin.
Structure Level Pattern Image Note
TS22: cochlea Present Regionally restricted 2A' , 2A" Expression in single row of cells located near medio-lateral border of organ of Corti, just lateral to developing inner hair cells. Authors state cells located in this position will develop as inner pllar cells. Lower levels of expression in developing outer hair cells region. Similar levels of expression in cells located lateral to the sensory epithelium. Authors state these cells will develop as Hensen's and Claudius cells and the outer sulcus.

Specimen 2B/2B': embryonic day 14.5 (more )
Note: Double labeled: pink - Gsk3b; green - Myo7a (assay MGI:6364593).
Structure Level Pattern Image Note
TS22: cochlea Present Regionally restricted 2B' , 2B Expression at apical surface of epithelium. Peak intensity of expression corresponds to inner pillar cells and Hensen's/Claudius/outer sulcus cells.

Specimen 2C'/2C": embryonic day 18.5 (more )
Note: Double labeled: pink - Gsk3b; green - F-actin.
Structure Level Pattern Image Note
TS26: organ of Corti Present Regionally restricted 2C" , 2C' Medial expression in inner pillar cells. Weaker expression in outer pillar cells, inner phalangeal cells and border cells. More intense expression in restricted group of cells lateral to sensory epithelium. Expression in Hensen's and Claudius cells.

Specimen 2D/2D': embryonic day 18.5 (more )
Note: Double labeled: pink - Gsk3b; green - Myo7a (assay MGI:6364593).
Structure Level Pattern Image Note
TS26: organ of Corti Present Regionally restricted 2D' , 2D Peak intensity of expression corresponds to inner pilar cells and Hensen's/Claudius cells.

Specimen 2E'/2E": postnatal day 0 (more )
Note: Double labeled: pink - Gsk3b; green - F-actin.
Structure Level Pattern Image Note
TS27: organ of Corti Present Regionally restricted 2E" , 2E' Expression markedly decreased overall but detected in inner pillar cells, Hensen's and Claudius cells.

Specimen 2F/2F': postnatal day 0 (more )
Note: Double labeled: pink - Gsk3b; green - Myo7a (assay MGI:6364593).
Structure Level Pattern Image Note
TS27: organ of Corti Present Regionally restricted 2F , 2F' Expression at apical surface of epithelium.


Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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last database update
05/28/2024
MGI 6.13
The Jackson Laboratory