Gene Expression Data
Assay Details
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Assay
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Reference:
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J:96667
Philips GT, et al., Precocious retinal neurons: Pax6 controls timing of differentiation and determination of cell type. Dev Biol. 2005 Mar 15;279(2):308-21
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6343361
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Gene symbol:
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Tubb3
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Gene name:
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tubulin, beta 3 class III
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Results
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Specimen
2A:
(close  )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup |
Present |
Regionally restricted |
2A
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Expression in optic vesicle. Some expressing cells have an elongated morphology typical of neurons in the earliest stage of differentiation, with processes spanning thickness of wall of optic vesicle.
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Specimen
2B:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup |
Present |
Regionally restricted |
2B
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Expression in optic vesicle. Expression in numerous cells with rounded cell bodies at outer (pial) surface.
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Specimen
2C:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup |
Present |
Regionally restricted |
2C
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Expression in optic vesicle. Expression in cells with neurite projections along pial surface.
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Specimen
3A:
embryonic day 9.5
(more  )
Note: 27 somites
Specimen
3A:
(close )
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Genetic Background:
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involves: FVB/N
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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27 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: optic vesicle |
Absent |
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3A
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No expressing neurons from E9.5 (27 somites) through E10.5 (34-38 somites).
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Specimen
Pax+/+ 34 somites not shown:
embryonic day 10.0
(more )
Specimen
Pax+/+ 34 somites not shown:
(close )
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Genetic Background:
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involves: FVB/N
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS16: optic cup |
Absent |
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No expressing neurons from E9.5 (27 somites) through E10.5 (34-38 somites).
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Specimen
3B:
embryonic day 10.5
(more )
Note: 36 somites
Specimen
3B:
(close )
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Genetic Background:
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involves: FVB/N
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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36 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup |
Absent |
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3B
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No expressing neurons from E9.5 (27 somites) through E10.5 (34-38 somites).
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Specimen
3C:
embryonic day 11.0
(more )
Note: 40 somites
Specimen
3C:
(close )
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Genetic Background:
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involves: FVB/N
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Age:
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embryonic day 11.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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40 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS18: neural retina |
Trace |
Regionally restricted |
3C
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Expression in one or a few neurons in central optic cup.
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Specimen
3D:
embryonic day 11.5
(more )
Note: 46 somites
Specimen
3D:
(close )
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Genetic Background:
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involves: FVB/N
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Age:
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embryonic day 11.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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46 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural retina |
Present |
Regionally restricted |
3D
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Rapid increase in number of expressing neurons.
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Specimen
3E:
embryonic day 9.5; Pax6Sey/ Pax6Sey
(more )
Note: 27 somites
Specimen
3E:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 9.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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27 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS15: optic vesicle |
Present |
Regionally restricted |
3E
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Expression in neurons occured precociously, appearing a day earlier than in wildtype.
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Specimen
Pax6-/- 30-64 somites not shown:
embryonic day 10.0; Pax6Sey/ Pax6Sey
(more )
Specimen
Pax6-/- 30-64 somites not shown:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Specimen
3F:
embryonic day 10.5; Pax6Sey/ Pax6Sey
(more )
Note: 36 somites
Specimen
3F:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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36 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS17: optic cup |
Present |
Regionally restricted |
3F
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Increased number of expressing neurons in the distal optic vesicle.
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Specimen
3G:
embryonic day 11.0; Pax6Sey/ Pax6Sey
(more )
Note: 40 somites
Specimen
3G:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 11.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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40 somites
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Specimen
3H:
embryonic day 11.5; Pax6Sey/ Pax6Sey
(more )
Note: 46 somites
Specimen
3H:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 11.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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46 somites
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Specimen
Pax6+/- 46 somites not shown:
embryonic day 11.5; Pax6Sey/Pax6 +
(more )
Specimen
Pax6+/- 46 somites not shown:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6+
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Age:
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embryonic day 11.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS19: neural retina |
Present |
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Authors state neurogenesis in optic vesicles did not begin in Pax6+/- embryos until 46 somite stage.
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Specimen
7A:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Double labeled: green - Tubb3; red - Dcx (assay MGI:6343364).
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Specimen
7B:
embryonic day 10.5; Pax6Sey/ Pax6Sey
(more )
Note: Double labeled: green - Tubb3; red - Nefm (assay MGI:6343365).
Specimen
7B:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Double labeled: green - Tubb3; red - Nefm (assay MGI:6343365).
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Specimen
7C:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Double labeled: green - Tubb3; red - Dlx1 (assay MGI:6343366); Dlx2 assay MGI:6343367).
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Specimen
7D:
embryonic day 10.5; Pax6Sey/ Pax6Sey
(more )
Note: Double labeled: green - Tubb3; red - Isl1 (assay MGI:6343368).
Specimen
7D:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Double labeled: green - Tubb3; red - Isl1 (assay MGI:6343368).
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Specimen
7E:
embryonic day 10.5; Pax6Sey/ Pax6Sey
(more )
Note: Double labeled: green - Tubb3; red - Pou4f1 (assay MGI:6343369).
Specimen
7E:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Double labeled: green - Tubb3; red - Pou4f1 (assay MGI:6343369).
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Specimen
7F:
embryonic day 10.5; Pax6Sey/ Pax6Sey
(more )
Note: Double labeled: green - Tubb3; red - Pou4f2 (assay MGI:6343370).
Specimen
7F:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Double labeled: green - Tubb3; red - Pou4f2 (assay MGI:6343370).
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Specimen
7G:
embryonic day 10.5; Pax6Sey/ Pax6Sey
(more )
Note: Double labeled: green - Tubb3; red - Stx1a (assay MGI:6343371).
Specimen
7G:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Double labeled: green - Tubb3; red - Stx1a (assay MGI:6343371).
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Specimen
7H:
embryonic day 10.5; Pax6Sey/ Pax6Sey
(more )
Note: Double labeled: green - Tubb3; red - VC1.1.
Specimen
7H:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 10.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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Double labeled: green - Tubb3; red - VC1.1.
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Specimen
8C:
embryonic day 14.5; Pax6Sey/ Pax6Sey
(more )
Note: 60 somites
Specimen
8C:
(close )
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Genetic Background:
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involves: FVB/N
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Mutant Allele(s):
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Pax6Sey/Pax6Sey
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Age:
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embryonic day 14.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Gelatin
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Note:
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60 somites
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: eye |
Trace |
Single cells |
8C
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Expression in single neuron in section of entire optic remnant.
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Analysis Tools