Gene Expression Data
Assay Details
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Assay
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Reference:
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J:32017
Offield MF, et al., PDX-1 is required for pancreatic outgrowth and differentiation of the rostral duodenum. Development. 1996 Mar;122(3):983-95
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6316652
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Gene symbol:
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Ins1
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Gene name:
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insulin I
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Antibody:
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anti-insulin
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Detection system:
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horseradish peroxidase
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Results
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Specimen
5G:
embryonic day 16.5; Pdx1tm2Cvw/Pdx1 +
(more  )
Note: Double labeled: brown - Ins1 and Ins2; blue - Pdx1 reporter (assay MGI:6316636).
Specimen
5G:
(close  )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Pdx1tm2Cvw/Pdx1+
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - Ins1 and Ins2; blue - Pdx1 reporter (assay MGI:6316636).
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Specimen
5H:
embryonic day 18.5; Pdx1tm2Cvw/Pdx1 +
(more )
Specimen
5H:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Pdx1tm2Cvw/Pdx1+
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Specimen
5K:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Pdx1tm2Cvw/Pdx1tm2Cvw
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: brown - Ins1 and Ins2; blue - Pdx1 reporter (assay MGI:6316636).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS24: duodenum |
Absent |
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5K
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Authors report no expression in dorsal ductules or malformed rostral duodenum.
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| TS24: pancreas |
Absent |
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5K
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Authors report no expression in dorsal ductules or malformed rostral duodenum.
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Specimen
5L:
(close )
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Genetic Background:
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involves: 129S1/Sv * 129X1/SvJ * Black Swiss
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Mutant Allele(s):
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Pdx1tm2Cvw/Pdx1tm2Cvw
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Age:
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embryonic day 18.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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| Structure |
Level |
Pattern |
Image |
Note |
| TS26: duodenum |
Absent |
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5L
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Authors report no expression in dorsal ductules or malformed rostral duodenum.
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| TS26: pancreas |
Absent |
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5L
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Authors report no expression in dorsal ductules or malformed rostral duodenum.
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