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Gene Expression Data
Assay Details
Assay
Reference: J:32017 Offield MF, et al., PDX-1 is required for pancreatic outgrowth and differentiation of the rostral duodenum. Development. 1996 Mar;122(3):983-95
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6316649
Gene symbol: Gcg
Gene name: glucagon
Antibody: anti-glucagon
Detection system: horseradish peroxidase
Results
Specimen 5A: embryonic day 9.5; Pdx1tm2Cvw/Pdx1+ (more )
Note: Double labeled: brown - Glu (Gcg); blue - Pdx1 reporter (assay MGI:6316636).
Structure Level Pattern Image Note
TS15: pancreas primordium Present Regionally restricted 5A Expression at in small clusters of cells at the endoderm/mesenchyme boundary and dispersed within the pancreatic bud.
Specimen 5B: embryonic day 9.5; Pdx1tm2Cvw/Pdx1tm2Cvw (more )
Note: Double labeled: brown - Glu (Gcg); blue - Pdx1 reporter (assay MGI:6316636).
Structure Level Pattern Image Note
TS15: pancreas primordium Present Regionally restricted 5B Authors report expression in similar populations as seen in +/- embryos.
Specimen 5C: embryonic day 11.5; Pdx1tm2Cvw/Pdx1+ (more )
Note: Double labeled: brown - Glu (Gcg); blue - Pdx1 reporter (assay MGI:6316636).
Structure Level Pattern Image Note
TS19: pancreas primordium Present 5C Authors report larger numbers of expressing cells. Expression within pancreatic buds and in clusters at the bud periphery.
Specimen 5D: embryonic day 11.5; Pdx1tm2Cvw/Pdx1tm2Cvw (more )
Note: Double labeled: brown - Glu (Gcg); blue - Pdx1 reporter (assay MGI:6316636).
Structure Level Pattern Image Note
TS19: pancreas primordium Present Regionally restricted 5D No expression in distal regions of ductule.
Specimen -/- E11.5 not shown: embryonic day 11.5; Pdx1tm2Cvw/Pdx1tm2Cvw (more )
Structure Level Pattern Image Note
TS19: duodenum Present Regionally restricted Expression in small number of cells at, or close to, junction of dorsal ductule with duodenal lumen.
Specimen 5E: embryonic day 16.5; Pdx1tm2Cvw/Pdx1+ (more )
Note: Double labeled: brown - Glu (Gcg); blue - Pdx1 reporter (assay MGI:6316636).
Structure Level Pattern Image Note
TS24: islets of Langerhans Present 5E Expression in budding islets.
Specimen 5I: embryonic day 16.5; Pdx1tm2Cvw/Pdx1tm2Cvw (more )
Note: Double labeled: brown - Glu (Gcg); blue - Pdx1 reporter (assay MGI:6316636).
Structure Level Pattern Image Note
TS24: pancreas epithelium Present Regionally restricted 5I Expression within epithelium of ductules.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory