Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:123725
Lax S, et al., CD248/Endosialin is dynamically expressed on a subset of stromal cells during lymphoid tissue development, splenic remodeling and repair. FEBS Lett. 2007 Jul 24;581(18):3550-6
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:6314775
|
Gene symbol:
|
Cd248
|
Gene name:
|
CD248 antigen, endosialin
|
Antibody:
|
P13
|
Detection system:
|
Secondary antibody
|
|
Results
|
Specimen
1A E16:
embryonic day 16.0
(more )
Note: Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
Specimen
1A E16:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 16.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spleen |
Strong |
Regionally restricted |
1A E16
|
Expression is mostly in the red pulp.
|
Specimen
1A Day 2:
postnatal day 2
(more )
Note: Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
Specimen
1A Day 2:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: spleen |
Present |
|
1A Day 2
|
|
Specimen
1A 2 week:
postnatal week 2
(more )
Note: Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
Specimen
1A 2 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spleen |
Present |
|
1A 2 week
|
|
Specimen
1A 6 week:
postnatal week 6
(more )
Note: Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
Specimen
1A 6 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spleen |
Present |
Regionally restricted |
1A 6 week
|
Expression is predominantly on vascular-like cells.
|
TS28: spleen red pulp |
Weak |
Regionally restricted |
1A 6 week
|
Expression is on interstitial fibroblasts.
|
Specimen
1B E16:
embryonic day 16.0
(more )
Note: Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
Specimen
1B E16:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 16.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: thymus |
Strong |
Regionally restricted |
1B E16
|
Expression is in developing vascular-like structures within the epithelial medulla and the outer layer of mesenchymal cells.
|
Specimen
1B Day 2:
postnatal day 2
(more )
Note: Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
Specimen
1B Day 2:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: thymus |
Present |
|
1B Day 2
|
|
Specimen
1B 2 week:
postnatal week 2
(more )
Note: Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
Specimen
1B 2 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: thymus |
Present |
|
1B 2 week
|
|
Specimen
1B 6 week:
postnatal week 5
(more )
Note: Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
Specimen
1B 6 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: thymus |
Weak |
|
1B 6 week
|
|
Specimen
1C E16:
embryonic day 16.0
(more )
Note: Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
Specimen
1C E16:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 16.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: hemolymphoid system |
Strong |
Regionally restricted |
1C E16
|
Expression is mostly confined to the outer mesenchymal layers of cells of the lymph node.
|
Specimen
1C Day 2:
postnatal day 2
(more )
Note: Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
Specimen
1C Day 2:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
|
Specimen
1C 2 week:
postnatal week 2
(more )
Note: Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
Specimen
1C 2 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
|
Specimen
1C 6 week:
postnatal week 6
(more )
Note: Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
Specimen
1C 6 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; gray - Cd3e (assay MGI:6314777).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lymph node |
Present |
Regionally restricted |
1C 6 week
|
Expression is restricted to vascular like structures.
|
TS28: lymph node follicle |
Weak |
Regionally restricted |
1C 6 week
|
Expression is in fibroblasts in the interstitial follicular area.
|
Specimen
2A E16:
embryonic day 16.0
(more )
Note: Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
Specimen
2A E16:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 16.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spleen |
Present |
|
2A E16
|
|
Specimen
2A Day 2:
postnatal day 2
(more )
Note: Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
Specimen
2A Day 2:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: spleen |
Present |
|
2A Day 2
|
|
Specimen
2A 2 week:
postnatal week 2
(more )
Note: Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
Specimen
2A 2 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spleen |
Present |
|
2A 2 week
|
|
Specimen
2A 6 week:
postnatal week 6
(more )
Note: Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
Specimen
2A 6 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spleen |
Present |
|
2A 6 week
|
|
Specimen
2B E16:
embryonic day 16.0
(more )
Note: Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
Specimen
2B E16:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 16.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: thymus |
Present |
|
2B E16
|
|
Specimen
2B Day 2:
postnatal day 2
(more )
Note: Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
Specimen
2B Day 2:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: thymus |
Present |
|
2B Day 2
|
|
Specimen
2B 2 week:
postnatal week 2
(more )
Note: Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
Specimen
2B 2 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: thymus |
Present |
|
2B 2 week
|
|
Specimen
2B 6 week:
postnatal week 6
(more )
Note: Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
Specimen
2B 6 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: thymus |
Present |
|
2B 6 week
|
|
Specimen
2C E16:
embryonic day 16.0
(more )
Note: Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
Specimen
2C E16:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 16.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
|
Specimen
2C Day 2:
postnatal day 2
(more )
Note: Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
Specimen
2C Day 2:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
|
Specimen
2C 2 week:
postnatal week 2
(more )
Note: Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
Specimen
2C 2 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
|
Specimen
2C 6 week:
postnatal week 6
(more )
Note: Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
Specimen
2C 6 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Cd248; red - Pecam1 (assay MGI:6314779).
|
Specimen
3A E16:
embryonic day 16.0
(more )
Note: Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
Specimen
3A E16:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 16.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spleen |
Present |
Regionally restricted |
3A E16
|
Expression is in stromal cells.
|
Specimen
3A Day 2:
postnatal day 2
(more )
Note: Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
Specimen
3A Day 2:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: spleen |
Present |
Regionally restricted |
3A Day 2
|
|
Specimen
3A 2 week:
postnatal week 2
(more )
Note: Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
Specimen
3A 2 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spleen |
Present |
Regionally restricted |
3A 2 week
|
|
Specimen
3A 6 week:
postnatal week 6
(more )
Note: Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
Specimen
3A 6 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: spleen |
Present |
Regionally restricted |
3A 6 week
|
|
Specimen
3B E16:
embryonic day 16.0
(more )
Note: Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
Specimen
3B E16:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 16.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: thymus |
Present |
Regionally restricted |
3B E16
|
Expression is in the epithelial medulla and surrounding mesenchyme.
|
Specimen
3B Day 2:
postnatal day 2
(more )
Note: Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
Specimen
3B Day 2:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: thymus |
Present |
|
3B Day 2
|
|
Specimen
3B 2 week:
postnatal week 2
(more )
Note: Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
Specimen
3B 2 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: thymus |
Present |
|
3B 2 week
|
|
Specimen
3B 6 week:
postnatal week 6
(more )
Note: Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
Specimen
3B 6 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: thymus |
Present |
|
3B 6 week
|
|
Specimen
3C E16:
embryonic day 16.0
(more )
Note: Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
Specimen
3C E16:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 16.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: hemolymphoid system |
Present |
Regionally restricted |
3C E16
|
Expression is in the inner medulla of the lymph node.
|
Specimen
3C Day 2:
postnatal day 2
(more )
Note: Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
Specimen
3C Day 2:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal day 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
|
Specimen
3C 2 week:
postnatal week 2
(more )
Note: Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
Specimen
3C 2 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 2
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
|
Specimen
3C 6 week:
postnatal week 6
(more )
Note: Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
Specimen
3C 6 week:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
postnatal week 6
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Acetone
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Cd248; red - Vcam1 (assay MGI:6314780); blue - ER-TR7 (reticular fibroblast marker).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: lymph node medulla |
Present |
Regionally restricted |
3C 6 week
|
Expression is vascular.
|
TS28: lymph node B cell domain |
Present |
Regionally restricted |
3C 6 week
|
Expression is in a small single positive population within the B cell follicular zone.
|
|