Shh RNA in situ Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:149237 Perez-Balaguer A, et al., Shh dependent and independent maintenance of basal midbrain. Mech Dev. 2009 May-Jun;126(5-6):301-13
Assay type:	RNA in situ
MGI Accession ID:	MGI:6304335
Gene symbol:	Shh
Gene name:	sonic hedgehog

Probe:	Shh probe144
Probe preparation:	Antisense RNA
Assay notes:	The probe used selectively discriminated functional from non-functional Shh mRNA in the conditional mutant.

Results

1B ©

Specimen 1B: embryonic day 10.5 (more

)

Structure	Level	Pattern	Image	Note
TS17: midbrain-hindbrain junction	Present		1B
TS17: midbrain	Present		1B

1G ©

Specimen 1G: embryonic day 10.5; Shh^tm2Amc/Shh^tm2Amc, En1^tm2(cre)Wrst/En1⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS17: midbrain	Absent		1G
TS17: midbrain-hindbrain junction	Absent		1G

1D ©

Specimen 1D: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain	Present		1D
TS20: midbrain-hindbrain junction	Present		1D

1I ©

Specimen 1I: embryonic day 12.5; Shh^tm2Amc/Shh^tm2Amc, En1^tm2(cre)Wrst/En1⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS20: midbrain	Absent		1I
TS20: midbrain-hindbrain junction	Absent		1I

2A ©

Specimen 2A: embryonic day 12.5 (more

)

Structure	Level	Pattern	Image	Note
TS20: midbrain floor plate	Present		2A
TS20: midbrain lateral wall	Present	Regionally restricted	2A	Expression was detected in the basal plate.

2G ©

Specimen 2G: embryonic day 12.5; Shh^tm2Amc/Shh^tm2Amc, En1^tm2(cre)Wrst/En1⁺ (more

)
Note: Conditional mutant.

Structure	Level	Pattern	Image	Note
TS20: midbrain floor plate	Absent		2G
TS20: midbrain lateral wall	Absent		2G	Absent in the basal plate.

3E ©

Specimen 3E: embryonic day 10.5 (more

)
Note: Double labeled: blue - Shh; red - Fgf8 (assay MGI:6304437).

Structure	Level	Pattern	Image	Note
TS17: midbrain floor plate	Present		3E

3F ©

Specimen 3F: embryonic day 10.5; Shh^tm2Amc/Shh^tm2Amc, En1^tm2(cre)Wrst/En1⁺ (more

)
Note: Conditional mutant. Double labeled: blue - Shh; red - Fgf8 (assay MGI:6304437).

Structure	Level	Pattern	Image	Note
TS17: midbrain floor plate	Absent		3F

3G ©

Specimen 3G: embryonic day 12.5 (more

)
Note: Double labeled: blue - Shh; red - Fgf8 (assay MGI:6304437).

Structure	Level	Pattern	Image	Note
TS20: midbrain floor plate	Present		3G

3H ©

Specimen 3H: embryonic day 12.5; Shh^tm2Amc/Shh^tm2Amc, En1^tm2(cre)Wrst/En1⁺ (more

)
Note: Conditional mutant. Double labeled: blue - Shh; red - Fgf8 (assay MGI:6304437).

Structure	Level	Pattern	Image	Note
TS20: midbrain floor plate	Absent		3H

3I ©

Specimen 3I: embryonic day 10.5 (more

)
Note: Double labeled: blue - Shh; red - Wnt1 (assay MGI:6304438).

Structure	Level	Pattern	Image	Note
TS17: midbrain floor plate	Present		3I

3J ©

Specimen 3J: embryonic day 10.5; Shh^tm2Amc/Shh^tm2Amc, En1^tm2(cre)Wrst/En1⁺ (more

)
Note: Conditional mutant. Double labeled: blue - Shh; red - Wnt1 (assay MGI:6304438).

Structure	Level	Pattern	Image	Note
TS17: midbrain floor plate	Absent		3J

3K ©

Specimen 3K: embryonic day 12.5 (more

)
Note: Double labeled: blue - Shh; red - Wnt1 (assay MGI:6304438).

Structure	Level	Pattern	Image	Note
TS20: midbrain floor plate	Present		3K

3L ©

Specimen 3L: embryonic day 12.5; Shh^tm2Amc/Shh^tm2Amc, En1^tm2(cre)Wrst/En1⁺ (more

)
Note: Conditional mutant. Double labeled: blue - Shh; red - Wnt1 (assay MGI:6304438).

Structure	Level	Pattern	Image	Note
TS20: midbrain floor plate	Absent		3L

Contributing Projects: Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources Funding Information Warranty Disclaimer, Privacy Notice, Licensing, & Copyright Send questions and comments to User Support.	last database update 03/25/2025 MGI 6.24