Gene Expression Data
Assay Details
|
Assay
|
|
Reference:
|
J:63342
Kanno Y, et al., A cystatin-related gene, testatin/cresp, shows male-specific expression in germ and somatic cells from the initial stage of murine gonadal sex-differentiation. Int J Dev Biol. 1999 Nov;43(8):777-84
|
|
Assay type:
|
RNA in situ
|
|
MGI Accession ID:
|
MGI:6286108
|
|
Gene symbol:
|
Cst9
|
|
Gene name:
|
cystatin 9
|
|
|
Results
|
Specimen
4A:
postnatal adult
(more  )
Specimen
4A:
(close  )
|
Genetic Background:
|
ICR
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
| TS28: spermatogonium |
|
Present |
Regionally restricted |
4A
|
Expression in round cells (spermatogonia) in extreme periphery of seminiferous tubules.
|
| TS28: Sertoli cell |
Sertoli cell |
Present |
|
4A
|
Expression in radially elongated Sertoli cells.
|
Specimen
4C:
postnatal adult
(more )
Note: Double labeled: blue - Cst9; brown - Gcna (TRA98; assay MGI:7488148).
Specimen
4C:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
postnatal adult
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: blue - Cst9; brown - Gcna (TRA98; assay MGI:7488148).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS28: spermatogonium |
Present |
Regionally restricted |
4C
,
4E
,
4D
|
Authors report that not all spermatogonia that are labeled with TRA98 co-express testatin/cresp (Cst9).
|
Specimen
5A/B:
embryonic day 12.5
(more )
Note: Double labeled: blue - testatin/cresp (Cst9); green - SSEA-1 (Fut4; assay MGI:6286113).
Specimen
5A/B:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: blue - testatin/cresp (Cst9); green - SSEA-1 (Fut4; assay MGI:6286113).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: primary sex cord |
Present |
Regionally restricted |
5A
,
5B
|
Specific expression within testis cord. Authors report expression in somatic cells.
|
| TS20: germ cell of testis |
Present |
Regionally restricted |
5B
,
5A
|
Authors report expression in 20-25% of germ cells. Some, but not all, co-express testatin/cresp (Cst9).
|
Specimen
5B upper inset:
embryonic day 12.5
(more )
Note: Double labeled: blue - testatin/cresp (Cst9); green - SSEA-1 (Fut4; assay MGI:6286113).
Specimen
5B upper inset:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: blue - testatin/cresp (Cst9); green - SSEA-1 (Fut4; assay MGI:6286113).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: germ cell of testis |
Present |
Regionally restricted |
5B upper inset
|
Black arrowheads indicate germ cells are double labeled.
|
| TS20: testis |
Present |
Regionally restricted |
5B upper inset
|
White arrowheads indicate somatic cells only expressing testin/cresp (Cst9).
|
Specimen
5B lower inset:
embryonic day 12.5
(more )
Note: Double labeled: blue - testatin/cresp (Cst9); green - SSEA-1 (Fut4; assay MGI:6286113).
Specimen
5B lower inset:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 12.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
|
Note:
|
Double labeled: blue - testatin/cresp (Cst9); green - SSEA-1 (Fut4; assay MGI:6286113).
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS20: germ cell of testis |
Present |
Regionally restricted |
5B lower inset
|
Black arrows indicate SSEA-1 (Fut4)-expressing germ cells that do not express testin/cresp (Cst9).
|
Specimen
not shown:
embryonic day 11.5
(more )
Specimen
not shown:
(close )
|
Genetic Background:
|
ICR
|
|
Age:
|
embryonic day 11.5
|
|
Sex:
|
Male
|
|
Type:
|
section
|
|
Fixation:
|
10% Formalin
|
|
Embedding:
|
Paraffin
|
| Structure |
Level |
Pattern |
Image |
Note |
| TS19: gonad |
Present |
Regionally restricted |
|
Authors report similar expression in germ cells and somatic cells in male gonads at 11.5 dpc.
|
|
Analysis Tools