Gene Expression Data
Assay Details
Assay
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Reference:
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J:133713
Kuntz S, et al., Tex19, a mammalian-specific protein with a restricted expression in pluripotent stem cells and germ line. Stem Cells. 2008 Mar;26(3):734-44
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6285147
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Gene symbol:
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Pou5f1
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Gene name:
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POU domain, class 5, transcription factor 1
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Antibody:
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anti-Oct4
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Detection system:
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Secondary antibody coupled to Cy3
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Results
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Specimen
4F 2 cells:
embryonic day 1.0
(more )
Note: Double labeled: red - Pou5f1; green - Tex19.1 (assay MGI:6285153).
Specimen
4F 2 cells:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 1.0
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Pou5f1; green - Tex19.1 (assay MGI:6285153).
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Structure |
Level |
Pattern |
Image |
Note |
TS2: 2-cell stage embryo |
Present |
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4F upper merge
,
4F upper Oct4
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Predominant nuclear staining.
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Specimen
4F 8 cells:
embryonic day 2.5
(more )
Note: Double labeled: red - Pou5f1; green - Tex19.1 (assay MGI:6285153).
Specimen
4F 8 cells:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 2.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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optical section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Pou5f1; green - Tex19.1 (assay MGI:6285153).
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Structure |
Level |
Pattern |
Image |
Note |
TS3: 8-cell stage embryo |
Present |
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4F middle Oct4
,
4F middle merge
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Predominant nuclear staining.
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Specimen
4F blastocyst:
embryonic day 3.5
(more )
Note: Double labeled: red - Pou5f1; green - Tex19.1 (assay MGI:6285153).
Specimen
4F blastocyst:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 3.5
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Age Note:
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Age assigned by curator based on morphological criteria supplied by authors.
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Pou5f1; green - Tex19.1 (assay MGI:6285153).
|
Structure |
Level |
Pattern |
Image |
Note |
TS4: blastocyst |
Present |
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4F lower Oct4
,
4F lower merge
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Predominant nuclear staining.
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