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Gene Expression Data
Assay Details
Assay
Reference: J:195491 Jackson SJ, et al., Rapid and widespread suppression of self-renewal by microRNA-203 during epidermal differentiation. Development. 2013 May;140(9):1882-91
Assay type: RNA in situ
MGI Accession ID: MGI:6273489
Gene symbol: Mir203
Gene name: microRNA 203
Probe: Mir203 probe1
Probe preparation: labelled with digoxigenin
Visualized with: Fluorescein
Results
Specimen 1A/C: embryonic day 15.0 (more )
Note: Triple labeled: green - Mir203; red - phosphorylated histone H3 (PH3); yellow - Itgb4 (assay MGI:6273491).
Structure Level Pattern Image Note
TS23: epidermis Present Regionally restricted 1A , 1C Low levels of expression in proliferative (dividing cells) in suprabasal layers of epidermis. Highly expressed in non-dividing cells.
Specimen 1D: embryonic day 17.0 (more )
Note: Triple labeled: green - Mir203; red - phosphorylated histone H3 (PH3); yellow - Itgb4 (assay MGI:6273491).
Structure Level Pattern Image Note
TS25: epidermis Strong Regionally restricted 1D Uniformly high expression in all suprabasal cells.
Specimen 1E: postnatal day 6 (more )
Note: Double labeled: green - Mir203; red - Itgb4 (assay MGI:6273491).
Structure Level Pattern Image Note
TS28: epidermis suprabasal layer Strong Uniform 1E Uniformly high expression in all suprabasal cells.
TS28: epidermis basal layer Absent 1E Expression restricted from basal progenitor cells.
Specimen 1F: postnatal day 6 (more )
Note: Double labeled: green - Mir203; red - Itgb4 (assay MGI:6273491).
Structure Level Pattern Image Note
TS28: hair follicle bulge Absent 1F Expression restricted from bulge hair follicle stem cells.
TS28: epidermis suprabasal layer Strong Regionally restricted 1F Highly expressed in terminally differentiated cells located in adjacent layer.
Specimen 2A/C: postnatal day 6 (more )
Note: Triple labeled: green - Mir203; red - BrdU; white - Krt5 (assay MGI:6273492).
Structure Level Pattern Image Note
TS28: epidermis basal layer Present Regionally restricted 2A , 2C Expression repressed in both basal cells following symmetric cell division in the basal layer.
Specimen 2D/F: postnatal day 6 (more )
Note: Triple labeled: green - Mir203; red - BrdU; white - Krt5 (assay MGI:6273492).
Structure Level Pattern Image Note
TS28: epidermis basal layer Strong Regionally restricted 2D , 2F Expression rapidly induced in differentiating daughter cell upon asymmetric cell division.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
MGI 6.24 		The Jackson Laboratory