Pard3tm1c(KOMP)Wtsi
Targeted Allele Detail
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| Symbol: |
Pard3tm1c(KOMP)Wtsi |
| Name: |
par-3 family cell polarity regulator; targeted mutation 1c, Wellcome Trust Sanger Institute |
| MGI ID: |
MGI:6270373 |
| Gene: |
Pard3 Location: Chr8:127790643-128338767 bp, + strand Genetic Position: Chr8, 74.66 cM, cytoband E2
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| Alliance: |
Pard3tm1c(KOMP)Wtsi page
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| IMPC: |
Pard3 gene page |
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| Germline Transmission: |
Earliest citation of germline transmission:
J:267913
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| Parent Cell Line: |
JM8A1.N3 (ES Cell)
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| Strain of Origin: |
C57BL/6N-Atm1Brd
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| Project Collection: |
KOMP-CSD
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| Allele Type: |
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Targeted (Conditional ready) |
| Mutation: |
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Insertion
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Mutation details: The L1L2_Bact_P cassette was inserted at position 128096346 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by a neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 128098621. The critical exon(s) is/are thus flanked by loxP sites.A "conditional ready" (floxed) allele was created by flp recombinase expression in mice carrying this allele to remove the lacZ sequence and neo selection cassette, leaving loxP sites flanking the critical exon(s). Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml
(J:267913)
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| Original: |
J:267913 Liu WA, et al., PARD3 dysfunction in conjunction with dynamic HIPPO signaling drives cortical enlargement with massive heterotopia. Genes Dev. 2018 Jun 1;32(11-12):763-780 |
| All: |
5 reference(s) |
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Analysis Tools
