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Gene Expression Data
Assay Details
Assay
Reference: J:266915 Bonet F, et al., CCBE1 is required for coronary vessel development and proper coronary artery stem formation in the mouse heart. Dev Dyn. 2018 Oct;247(10):1135-1145
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6270371
Gene symbol: Cdh5
Gene name: cadherin 5
Antibody: clone 11D4-1
Detection system: Secondary antibody coupled to Alexa Fluor 488
Results
Specimen 2A WT E12.5: embryonic day 12.5 (more )
Note: Double labeled: green - Cdh5; red - alpha-actinin.
Structure Level Pattern Image Note
TS20: coronary vascular plexus Present 2A upper left , 2A upper middle
Specimen 2A KO E12.5: embryonic day 12.5; Ccbe1tm1Lex/Ccbe1tm1Lex (more )
Note: Double labeled: green - Cdh5; red - alpha-actinin.
Structure Level Pattern Image Note
TS20: coronary vascular plexus Present Regionally restricted 2A lower middle , 2A lower left Fewer branch points in severely underdeveloped dorsal subepicardial coronary vessels in mutant hearts, compared with the wild-type.
Specimen 2A WT E13.5: embryonic day 13.5 (more )
Note: Double labeled: green - Cdh5; red - alpha-actinin.
Structure Level Pattern Image Note
TS21: coronary artery Present 2A upper right
Specimen 2A KO E13.5: embryonic day 13.5; Ccbe1tm1Lex/Ccbe1tm1Lex (more )
Note: Double labeled: green - Cdh5; red - alpha-actinin.
Structure Level Pattern Image Note
TS21: coronary artery Present Regionally restricted 2A lower right Fewer branch points in severely underdeveloped dorsal subepicardial coronary vessels in mutant hearts, compared with the wild-type.
Specimen 3A WT: embryonic day 13.5 (more )
Note: Double labeled: green - Cdh5; red - Dach1 (assay MGI:6270387).
Structure Level Pattern Image Note
TS21: coronary artery Present 3A upper left
Specimen 3A KO: embryonic day 13.5; Ccbe1tm1Lex/Ccbe1tm1Lex (more )
Note: Double labeled: green - Cdh5; red - Dach1 (assay MGI:6270387).
Structure Level Pattern Image Note
TS21: coronary artery Present Regionally restricted 3A upper right Severely underdeveloped dorsal intramyocardial coronary vessels were stunted in mutant hearts.
Specimen 4A WT: embryonic day 14.0 (more )
Note: Double labeled: green - Cdh5; red - Dach1 (assay MGI:6270387).
Structure Level Pattern Image Note
TS22: coronary artery Present 4A upper left
Specimen 4A KO: embryonic day 14.0; Ccbe1tm1Lex/Ccbe1tm1Lex (more )
Note: Double labeled: green - Cdh5; red - Dach1 (assay MGI:6270387).
Structure Level Pattern Image Note
TS22: coronary artery Present Regionally restricted 4A upper right Ventral intramyocardial coronary vessels were underdeveloped in mutant hearts, also displaying fewer endothelial vesicles on the ventral side of the interventricular septum.
Specimen 4C WT: embryonic day 14.0 (more )
Note: Double labeled: green - Cdh5; red - Dach1 (assay MGI:6270387).
Structure Level Pattern Image Note
TS22: coronary artery Present 4C upper left
Specimen 4C KO: embryonic day 14.0; Ccbe1tm1Lex/Ccbe1tm1Lex (more )
Note: Double labeled: green - Cdh5; red - Dach1 (assay MGI:6270387).
Structure Level Pattern Image Note
TS22: coronary artery Present Regionally restricted 4C upper right Fewer subepicardial and intramyocardial coronary vessels in mutant hearts, also displaying fewer endothelial vesicles on the ventral side of the interventricular septum.
Specimen not shown: embryonic day 14.0; Ccbe1tm1Lex/Ccbe1tm1Lex (more )
Structure Level Pattern Image Note
TS22: coronary artery Present Regionally restricted Fewer branch points in severely underdeveloped dorsal subepicardial coronary vessels in mutant hearts, compared with the wild-type.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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03/25/2025
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