Gene Expression Data
Assay Details
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Assay
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Reference:
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J:266571
Minaki Y, et al., Migrating postmitotic neural precursor cells in the ventricular zone extend apical processes and form adherens junctions near the ventricle in the developing spinal cord. Neurosci Res. 2005 Jul;52(3):250-62
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Assay type:
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RNA in situ
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MGI Accession ID:
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MGI:6259683
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Gene symbol:
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Dll1
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Gene name:
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delta like canonical Notch ligand 1
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Probe:
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Dll1 cDNA10
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Probe preparation:
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Antisense labelled with digoxigenin RNA
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Visualized with:
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Alkaline phosphatase
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Assay notes:
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For most of the specimens a fast red substrate was used for visualization.
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Results
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Specimen
2E:
embryonic day 12.5
(more  )
Note: Double labeled: red - Dll1; purple - Kirrel2 (assay MGI:6259679).
Specimen
2E:
(close  )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Dll1; purple - Kirrel2 (assay MGI:6259679).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord ventricular layer |
Present |
Regionally restricted |
2F Dll1/Neph3
,
2E Dll1
,
2F Dll1
,
2E Dll1/Neph3
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Expression is in post-mitotic cells.
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Specimen
6A:
embryonic day 12.5
(more )
Note: Double labeled: red - Dll1; green - Tjp1 (assay MGI:6259688).
Specimen
6A:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Dll1; green - Tjp1 (assay MGI:6259688).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord ventricular layer |
Present |
Regionally restricted |
6B Dll1
,
6B merge
,
6A merge
,
6A Dll1
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Expression is in the cell bodies and the apically extending processes and accumulates at the process termini adjacent to the luminal surface.
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| TS20: spinal cord mantle layer |
Absent |
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6A Dll1
,
6A merge
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Specimen
6C:
embryonic day 12.5
(more )
Note: Double labeled: red - Dll1; green - Tjp1 (assay MGI:6259688).
Specimen
6C:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Dll1; green - Tjp1 (assay MGI:6259688).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord ventricular layer |
Present |
Regionally restricted |
6C
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Expression is in the cell bodies and the apically extending processes and accumulates at the process termini adjacent to the luminal surface.
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Specimen
6D:
embryonic day 12.5
(more )
Note: Double labeled: red - Dll1; green - Tjp1 (assay MGI:6259688).
Specimen
6D:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Dll1; green - Tjp1 (assay MGI:6259688).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: spinal cord ventricular layer |
Present |
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6D
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Expression is in the cell bodies and the apically extending processes and accumulates at the process termini adjacent to the luminal surface.
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Specimen
6E:
embryonic day 12.5
(more )
Note: Double labeled: red - Dll1; green - Tjp1 (assay MGI:6259688).
Specimen
6E:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Dll1; green - Tjp1 (assay MGI:6259688).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: telencephalon ventricular layer |
Present |
Regionally restricted |
6E merge
,
6E Dll1
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Expression is in the cell bodies and the apically extending processes and accumulates at the process termini adjacent to the luminal surface.
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Specimen
6F:
embryonic day 12.5
(more )
Note: Double labeled: red - Dll1; green - Tjp1 (assay MGI:6259688).
Specimen
6F:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: red - Dll1; green - Tjp1 (assay MGI:6259688).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: midbrain ventricular layer |
Present |
Regionally restricted |
6F Dll1
,
6F merge
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Expression is in the cell bodies and the apically extending processes and accumulates at the process termini adjacent to the luminal surface.
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Specimen
not shown:
embryonic day 12.5
(more )
Specimen
not shown:
(close )
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Genetic Background:
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Not Specified
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Age:
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embryonic day 12.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS20: metencephalon ventricular layer |
Present |
Regionally restricted |
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Expression is in the cell bodies and the apically extending processes and accumulates at the process termini adjacent to the luminal surface.
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