Cdh1 Immunohistochemistry Gene Expression Assay

Gene Expression Data
Assay Details

Assay

Reference:	J:258304 Pui HP, et al., NANOS2 acts as an intrinsic regulator of gonocytes-to-spermatogonia transition in the murine testes. Mech Dev. 2018 Feb;149:27-40
Assay type:	Immunohistochemistry
MGI Accession ID:	MGI:6257341
Gene symbol:	Cdh1
Gene name:	cadherin 1

Antibody:	anti-E-Cadherin
Detection system:	Secondary antibody

Results

1A E17.5 bottom ©

1A E17.5 middle ©

1A E17.5 top ©

Specimen 1A E17.5: embryonic day 17.5 (more

)
Note: Double labeled: white - Cdh1; green - Nanos2 (assay MGI:6257340).

Structure	Level	Pattern	Image	Note
TS25: germ cell of testis	Present		1A E17.5 middle , 1A E17.5 top , 1A E17.5 bottom

1A E18.5 bottom ©

1A E18.5 middle ©

1A E18.5 top ©

Specimen 1A E18.5: embryonic day 18.5 (more

)
Note: Double labeled: white - Cdh1; green - Nanos2 (assay MGI:6257340).

Structure	Level	Pattern	Image	Note
TS26: germ cell of testis	Present		1A E18.5 bottom , 1A E18.5 top , 1A E18.5 middle

1A P0.5 bottom ©

1A P0.5 middle ©

1A P0.5 top ©

Specimen 1A P0.5: postnatal day 0.5 (more

)
Note: Double labeled: white - Cdh1; green - Nanos2 (assay MGI:6257340).

Structure	Level	Pattern	Image	Note
TS27: germ cell of testis	Present		1A P0.5 bottom , 1A P0.5 top , 1A P0.5 middle

1A P1.5 bottom ©

1A P1.5 middle ©

1A P1.5 top ©

Specimen 1A P1.5: postnatal day 1.5 (more

)
Note: Double labeled: white - Cdh1; green - Nanos2 (assay MGI:6257340).

Structure	Level	Pattern	Image	Note
TS27: germ cell of testis	Present		1A P1.5 middle , 1A P1.5 top , 1A P1.5 bottom

6B I Control ©

Specimen 6B I Control: embryonic day 16.5 (more

)
Note: Double labeled: red - Cdh1; green - Phospho-Histone 3.

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		6B I Control

6B I N2KO ©

Specimen 6B I N2KO: embryonic day 16.5; Nanos2^tm1Ysa/Nanos2^tm1Ysa (more

)
Note: Double labeled: red - Cdh1; green - Phospho-Histone 3.

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		6B I N2KO

6B II Control ©

Specimen 6B II Control: embryonic day 16.5 (more

)
Note: Double labeled: red - Cdh1; green - Laminin.

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		6B II Control

6B II N2KO ©

Specimen 6B II N2KO: embryonic day 16.5; Nanos2^tm1Ysa/Nanos2^tm1Ysa (more

)
Note: Double labeled: red - Cdh1; green - Laminin.

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		6B II N2KO
TS24: interstitium of the testis	Present	Single cells	6B II N2KO	Expression is in germ cells and 40% were found in the interstitium.

6F II Control ©

Specimen 6F II Control: embryonic day 16.5 (more

)
Note: Double labeled: red - Cdh1; green - Zbtb16 (assay MGI:6257409).

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		6F II Control

6F II N2KO ©

Specimen 6F II N2KO: embryonic day 16.5; Nanos2^tm1Ysa/Nanos2^tm1Ysa (more

)
Note: Double labeled: red - Cdh1; green - Zbtb16 (assay MGI:6257409).

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		6F II N2KO

6F III Control ©

Specimen 6F III Control: embryonic day 16.5 (more

)
Note: Double labeled: red - Cdh1; green - Foxo1 (assay MGI:6257410).

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		6F III Control

6F III N2KO ©

Specimen 6F III N2KO: embryonic day 16.5; Nanos2^tm1Ysa/Nanos2^tm1Ysa (more

)
Note: Double labeled: red - Cdh1; green - Foxo1 (assay MGI:6257410).

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		6F III N2KO

Specimen S6 I Control: embryonic day 16.5 (more

)
Note: Double labeled: red - Cdh1; green - Laminin.

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		S6_I Control second , S6_I Control ECAD , S6_I Control left

S6_II 1 ©

S6_II 2 ©

S6_II 3 ©

S6_II left ©

Specimen S6 II N2KO: embryonic day 16.5; Nanos2^tm1Ysa/Nanos2^tm1Ysa (more

)
Note: Double labeled: red - Cdh1; green - Laminin.

Structure	Level	Pattern	Image	Note
TS24: germ cell of testis	Present		S6_II ECAD middle , S6_II 3 , S6_II ECAD bottom , S6_II left , S6_II 2 , S6_II ECAD top , S6_II 1	There are cytoplasmic projections of gonocytes on the outer surface of seminiferous cords.
TS24: interstitium of the testis	Present	Single cells	S6_II 2 , S6_II ECAD middle , S6_II 1 , S6_II ECAD top , S6_II left , S6_II ECAD bottom , S6_II 3	Expression is in germ cells in the interstitium.

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