Gene Expression Data
Assay Details
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Assay
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Reference:
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J:263979
Harley RJ, et al., Neuronal cell adhesion molecule (NrCAM) is expressed by sensory cells in the cochlea and is necessary for proper cochlear innervation and sensory domain patterning during development. Dev Dyn. 2018 Mar 14;
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6256953
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Gene symbol:
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Sox2
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Gene name:
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SRY (sex determining region Y)-box 2
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Antibody:
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anti-Sox2
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Detection system:
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Secondary antibody coupled to Cy5
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Results
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Specimen
4D:
embryonic day 14.5
(more  )
Note: Double labeled: purple - Sox2; green - Tubb3 (assay MGI:6241344).
Specimen
4D:
(close  )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: purple - Sox2; green - Tubb3 (assay MGI:6241344).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlea |
Present |
Regionally restricted |
4D
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Expression is in the prosensory domain.
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Specimen
4E:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Nrcamtm1Gmt/Nrcamtm1Gmt
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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Note:
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Double labeled: purple - Sox2; green - Tubb3 (assay MGI:6241344).
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlea |
Present |
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4E
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Expression in this mutant is the same as in wild-type.
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Specimen
4G:
embryonic day 14.5
(more )
Specimen
4G:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlea |
Present |
Regionally restricted |
4G
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Expression is in the prosensory domain and in Schwann cells.
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Specimen
4H:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Nrcamtm1Gmt/Nrcamtm1Gmt
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Age:
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embryonic day 14.5
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS22: cochlea |
Present |
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4H
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Expression in this mutant is the same as in wild-type.
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Specimen
5G:
postnatal day 0
(more )
Specimen
5G:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlea |
Present |
Regionally restricted |
5G
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Expression is in supporting cells.
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Specimen
5H:
postnatal day 0; Nrcamtm1Gmt/Nrcam +
(more )
Specimen
5H:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Nrcamtm1Gmt/Nrcam+
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlea |
Present |
Regionally restricted |
5H
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Expression is in supporting cells.
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Specimen
5I:
(close )
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Genetic Background:
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involves: 129S6/SvEvTac * C57BL/6
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Mutant Allele(s):
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Nrcamtm1Gmt/Nrcamtm1Gmt
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Age:
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postnatal day 0
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Sex:
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Not Specified
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Type:
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whole mount
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Fixation:
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4% Paraformaldehyde
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| Structure |
Level |
Pattern |
Image |
Note |
| TS27: cochlea |
Present |
Regionally restricted |
5I
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Expression is in supporting cells. This mutant has a patterning defect and irregular-shaped cells.
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Analysis Tools