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Gene Expression Data
Assay Details
Assay
Reference: J:191199 Mulvaney JF, et al., Secreted Factor R-Spondin 2 is Involved in Refinement of Patterning of the Mammalian Cochlea. Dev Dyn. 2013 Feb;242(2):179-88
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6241482
Gene symbol: Nefm
Gene name: neurofilament, medium polypeptide
Antibody: anti-neurofilament clone 2H3
Detection system: Secondary antibody
Results
Specimen 5A/D: embryonic day 18.5 (more )
Note: Base area. Double labeled: red - Nefm; green - Myo6 (assay MGI:6241481).
Structure Level Pattern Image Note
TS26: vestibulocochlear nerve cochlear component Present 5A , 5D Expressed in the nerve fibers, which entered the sensory epithelium and uniformly turn towards the base, forming three clearly defined tracks.
Specimen 5B/E: embryonic day 18.5 (more )
Note: Mid base area. Double labeled: red - Nefm; green - Myo6 (assay MGI:6241481).
Structure Level Pattern Image Note
TS26: vestibulocochlear nerve cochlear component Present 5E , 5B Expressed in the nerve fibers, which ntered the sensory epithelium and turned towards the base and are in the process of forming bundles.
Specimen 5C/F: embryonic day 18.5 (more )
Note: Apex area. Double labeled: red - Nefm; green - Myo6 (assay MGI:6241481).
Structure Level Pattern Image Note
TS26: vestibulocochlear nerve cochlear component Present 5F , 5C Expressed in the nerve fibers, which entered the sensory epithelium and turned towards the base but have not yet started to form bundles.
Specimen 5G/J: embryonic day 18.5; Rspo2tm1Nuv/Rspo2tm1Nuv (more )
Note: Base area. Double labeled: red - Nefm; green - Myo6 (assay MGI:6241481).
Structure Level Pattern Image Note
TS26: vestibulocochlear nerve cochlear component Present 5G , 5J Expressed in the nerve fibers, which entered the sensory epithelium, but not formed tracks and some appear to be turning towards the apex.
Specimen 5H/K: embryonic day 18.5; Rspo2tm1Nuv/Rspo2tm1Nuv (more )
Note: Mid base area. Double labeled: red - Nefm; green - Myo6 (assay MGI:6241481).
Structure Level Pattern Image Note
TS26: vestibulocochlear nerve cochlear component Present 5K , 5H Expressed in the nerve fibers, which were disorganized.
Specimen 5I/L: embryonic day 18.5; Rspo2tm1Nuv/Rspo2tm1Nuv (more )
Note: Apex area. Double labeled: red - Nefm; green - Myo6 (assay MGI:6241481).
Structure Level Pattern Image Note
TS26: vestibulocochlear nerve cochlear component Present 5I , 5L Expressed in the nerve fibers, which were disorganized.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/07/2024
MGI 6.23 		The Jackson Laboratory