Probe:	Rspo2 probe2
Probe preparation:	Antisense labelled with digoxigenin RNA
Visualized with:	Alkaline phosphatase

Structure	Level	Pattern	Image	Note
TS22: cochlea epithelium	Present	Regionally restricted	1E , 1A , 1M , 1I	Expressed in the basal greater epithelial ridge.

Structure	Level	Pattern	Image	Note
TS23: cochlea epithelium	Present	Regionally restricted	1N , 1F , 1J , 1B	Expression extended from the base to the mid base (around 65% of the length of the cochlea).

Structure	Level	Pattern	Image	Note
TS25: cochlea epithelium	Present	Regionally restricted	1K , 1O , 1C , 1G	Expression had expanded to the apex, was maintained in the mid base, and began to downregulate in the base.

Structure	Level	Pattern	Image	Note
TS27: cochlea epithelium	Present	Regionally restricted	1L , 1D , 1P , 1H	Expression was restricted to the apex and apical portion of the mid base.

Structure	Level	Pattern	Image	Note
TS25: cochlea epithelium	Present	Regionally restricted	1R , 1S , 1T , 1Q	Expressed in a region around 75um medial to the organ of Corti in the greater epithelial ridge.

Structure	Level	Pattern	Image	Note
TS25: cochlear duct	Present	Regionally restricted	1U	Expressed in cells of the inner sulcus. Expressed in the luminal surface cells of the most lateral region of the greater epithelial ridge.

Structure	Level	Pattern	Image	Note
TS25: cochlear duct	Present	Regionally restricted	1V	Expressed in cells of the inner sulcus. In a less mature region of the cochlea, expression spaned the depth of the greater epithelial ridge and was more diffuse.

Structure	Level	Pattern	Image	Note
TS21: cochlea	Absent