Gene Expression Data
Assay Details
Assay
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Reference:
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J:259135
Grifone R, et al., The RNA-binding protein Rbm24 is transiently expressed in myoblasts and is required for myogenic differentiation during vertebrate development. Mech Dev. 2014 Nov;134:1-15
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6202762
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Gene symbol:
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Pax3
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Gene name:
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paired box 3
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Antibody:
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anti-Pax3
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Detection system:
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Secondary antibody coupled to Alexa Fluor 594
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Results
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Specimen
2G:
embryonic day 11.5
(more )
Note: Double labeled: red - Pax3; green - Rbm24 (assay MGI:6202765).
Specimen
2G:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Pax3; green - Rbm24 (assay MGI:6202765).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: dermomyotome |
Present |
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2G
|
|
TS19: myotome |
Present |
Single cells |
2G
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There are a few interspersed positive cells in the myotome.
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TS19: hindlimb bud |
Present |
Regionally restricted |
2G
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Expression is in migrating cells from the dermamyotome into the hindlimb.
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TS19: trunk mesenchyme |
Present |
Regionally restricted |
2G
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Expression is in migrating cells from the dermamyotome into the hindlimb.
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Specimen
2H:
embryonic day 11.5
(more )
Note: Double labeled: red - Pax3; green - Rbm24 (assay MGI:6202765).
Specimen
2H:
(close )
Genetic Background:
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C57BL/6
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Age:
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embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Note:
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Double labeled: red - Pax3; green - Rbm24 (assay MGI:6202765).
|
Specimen
2I:
embryonic day 11.5
(more )
Note: Double labeled: red - Pax3; green - Rbm24 (assay MGI:6202765).
Specimen
2I:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pax3; green - Rbm24 (assay MGI:6202765).
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: dermomyotome |
Present |
|
2I
|
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TS19: forelimb bud |
Present |
Regionally restricted |
2I
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Expression is in migrating cells from the dermamyotome into the forelimb.
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TS19: trunk mesenchyme |
Present |
Regionally restricted |
2I
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Expression is in migrating cells from the dermamyotome into the forelimb.
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Specimen
2J:
embryonic day 11.5
(more )
Note: Double labeled: red - Pax3; green - Rbm24 (assay MGI:6202765).
Specimen
2J:
(close )
Genetic Background:
|
C57BL/6
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Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
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Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
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Paraffin
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Note:
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Double labeled: red - Pax3; green - Rbm24 (assay MGI:6202765).
|
Specimen
2K:
embryonic day 11.5
(more )
Note: Double labeled: red - Pax3; green - Rbm24 (assay MGI:6202765).
Specimen
2K:
(close )
Genetic Background:
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C57BL/6
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: red - Pax3; green - Rbm24 (assay MGI:6202765).
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Specimen
2L:
embryonic day 11.5
(more )
Note: Double labeled: red - Pax3; green - Rbm24 (assay MGI:6202765).
Specimen
2L:
(close )
Genetic Background:
|
C57BL/6
|
Age:
|
embryonic day 11.5
|
Sex:
|
Not Specified
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Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: red - Pax3; green - Rbm24 (assay MGI:6202765).
|
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