About   Help   FAQ
Gene Expression Data
Assay Details
Assay
Reference: J:264256 Bernardo AS, et al., Mammalian embryo comparison identifies novel pluripotency genes associated with the naive or primed state. Biol Open. 2018 Aug 17;7(8):bio033282
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6202660
Gene symbol: Pou5f1
Gene name: POU domain, class 5, transcription factor 1
Antibody: anti-Oct4 (N-19; sc-8628)
Detection system: Secondary antibody coupled to Alexa Fluor 594
Results
Specimen 5D mICM top: embryonic day 3.5 (more )
Note: Double labeled: red - Pou5f1; green - Gjb5 (assay MGI:6202661).
Structure Level Pattern Image Note
TS4: inner cell mass Present 5D Oct4 mICM top , 5D Merge mICM top
Specimen 5D mICM middle: embryonic day 3.5 (more )
Note: Double labeled: red - Pou5f1; green - Trip6 (assay MGI:6202662).
Structure Level Pattern Image Note
TS4: inner cell mass Present 5D Oct4 mICM middle , 5D Merge mICM middle
Specimen 5D mERSE top: embryonic day 6.25 (more )
Note: Double labeled: red - Pou5f1; green - Gjb5 (assay MGI:6202661). Either 129S2 or (C57BL/6J x DBA/2J)F1 mice were used. ERSE - epithelial radially symmetric epiblast.
Structure Level Pattern Image Note
TS8: epiblast Present 5D Oct4 mERSE top , 5D Merge mERSE top
Specimen 5D mERSE middle: embryonic day 6.25 (more )
Note: Double labeled: red - Pou5f1; green - Trip6 (assay MGI:6202662). Either 129S2 or (C57BL/6J x DBA/2J)F1 mice were used. ERSE - epithelial radially symmetric epiblast.
Structure Level Pattern Image Note
TS8: epiblast Present 5D Merge mERSE middle , 5D Oct4 mERSE middle
Specimen S5F left: embryonic day 3.5 (more )
Note: Double labeled: red - Pou5f1; green - Klf4 (assay MGI:6202677).
Structure Level Pattern Image Note
TS4: inner cell mass Present S5F Merge left , S5F Oct4 left
Specimen S5F right: embryonic day 6.25 (more )
Note: Double labeled: red - Pou5f1; green - Klf4 (assay MGI:6202677). Either 129S2 or (C57BL/6J x DBA/2J)F1 mice were used. ERSE - epithelial radially symmetric epiblast.
Structure Level Pattern Image Note
TS8: epiblast Present S5F Oct4 right , S5F Merge right
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
Citing These Resources
Funding Information
Warranty Disclaimer, Privacy Notice, Licensing, & Copyright
Send questions and comments to User Support. 		last database update
03/18/2025
MGI 6.24 		The Jackson Laboratory