Antibody:	FABP5 antibody
Detection system:	Alexa Fluor 488
Assay notes:	Two antibodies were described, but the one used in this assay was not detailed. Anti-Krt14 (MGI:6196068) may have been used in this assay.

Structure	Level	Pattern	Image	Note
TS21: back skin	Absent		2a	No expression in E13.5 skin.

Structure	Level	Pattern	Image	Note
TS23: epidermis	Present	Regionally restricted	2b	Expression in developing suprabasal layer of keratinocytes.

Structure	Level	Pattern	Image	Note
TS25: epidermis	Present	Regionally restricted	2c	Expression maintained in suprabasal epidermis. Expression largely absent from proliferating basal keratinocytes and upper-most differentiated layers of epidermis.

Structure	Level	Pattern	Image	Note
TS27: epidermis	Present	Regionally restricted	2d	Expression in suprabasal keratinocytes at all postnatal and adult stages examined. Expression in clusters of cells associated with tops of most mature primary follicles.

Structure	Level	Pattern	Image	Note
TS28: epidermis suprabasal layer	Present	Regionally restricted	2e	Expression in suprabasal keratinocytes at all postnatal and adult stages examined.

Structure	Level	Pattern	Image	Note
TS25: vibrissa follicle	Present	Regionally restricted	2f	Expression in clusters of cells associated with whisker follicles.

Structure	Level	Pattern	Image	Note
TS28: sebaceous gland	Present	Regionally restricted	2g	Expression demarcates more mature developing sebaceous glands.
TS28: hair bulb	Strong		2g
TS28: hair inner root sheath	Strong		2g

Structure	Level	Pattern	Image	Note
TS27: hair follicle	Present	Regionally restricted	2i	Expression in clusters of cells associated with tops of most mature primary follicles, appearing to be germinal sebaceous glands.

Structure	Level	Pattern	Image	Note
TS27: tail	Present	Regionally restricted	2j	Expression in clusters of cells associated with tops of most mature primary follicles, appearing to be germinal sebaceous glands.

Structure	Level	Pattern	Image	Note
TS28: sebaceous gland	Present	Regionally restricted	2k	Authors state that expressing cells located in the sebaceous gland region were not proliferative.

Structure	Level	Pattern	Image	Note
TS28: epidermis suprabasal layer	Present	Regionally restricted	2l	Expression in suprabasal keratinocytes at all postnatal and adult stages examined. Expression in majority of differentiated cells.
TS28: hair follicle	Ambiguous		2l	Weak or absent expression from adult telogen hair follicles.

Structure	Level	Pattern	Image	Note
TS28: epidermis suprabasal layer	Present	Regionally restricted	2m	Prominent nuclear localization of expression in cells at interface between differentiating suprabasal layer and proliferating basal layer.

Structure	Level	Pattern	Image	Note
TS28: hair follicle	Present	Regionally restricted	2n	Expression in adult anagen hair follicles similar in pattern and intensity to that seen at P5.
TS28: hair follicle dermal papilla	Absent		2n

Structure	Level	Pattern	Image	Note
TS28: back skin	Present	Regionally restricted	5a	Authors report pattern of expression essentially similar to that seen in telogen skin of 42-day-old animals.

Structure	Level	Pattern	Image	Note
TS27: tail	Present	Regionally restricted	5e