Gene Expression Data
Assay Details
Assay
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Reference:
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J:142873
Collins CA, et al., Dynamic regulation of retinoic acid-binding proteins in developing, adult and neoplastic skin reveals roles for beta-catenin and Notch signalling. Dev Biol. 2008 Dec 1;324(1):55-67
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6196078
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Gene symbol:
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Crabp1
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Gene name:
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cellular retinoic acid binding protein I
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Antibody:
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anti-CRABP1
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Detection system:
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Alexa Fluor 488
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Results
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Specimen
1b:
embryonic day 13.5
(more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Specimen
1b:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: back skin |
Present |
Regionally restricted |
1b
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Expression first seen at E13.5 during earliest stage of skin development, when epidermis consists of single layer of keratinocytes. Expression in cytoplasm of cells with fibroblast-type morphology, closely associated with basal surface.
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TS21: epidermis |
Absent |
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1b
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No expression in Krt14-expressing epidermal cells.
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Specimen
1c:
embryonic day 13.5
(more )
Note: Double labeled: green - Crabp1; red - Mki67 (assay MGI:6196083).
Specimen
1c:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Crabp1; red - Mki67 (assay MGI:6196083).
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Specimen
1d:
embryonic day 15.5
(more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Specimen
1d:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
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Specimen
1e:
embryonic day 15.5
(more )
Note: Double labeled: green - Crabp1; red - Mki67 (assay MGI:6196083).
Specimen
1e:
(close )
Genetic Background:
|
involves: C57BL/6 * CBA
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Age:
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embryonic day 15.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Crabp1; red - Mki67 (assay MGI:6196083).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: dermis |
Strong |
Regionally restricted |
1e
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Strong expression in dermal condensate.
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Specimen
1f:
embryonic day 17.5
(more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Specimen
1f:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: hair follicle dermal papilla |
Present |
Regionally restricted |
1f
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Strong expression in dermal papilla of new secondary hair pegs. Lower intensity of expression in dermal papilla of more mature primary follicles.
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Specimen
1g:
embryonic day 17.5
(more )
Note: Double labeled: green - Crabp1; red - Mki67 (assay MGI:6196083).
Specimen
1g:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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embryonic day 17.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Crabp1; red - Mki67 (assay MGI:6196083).
|
Structure |
Level |
Pattern |
Image |
Note |
TS25: dermis |
Present |
Regionally restricted |
1g
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Expression maintained in dermal condensate, in cells concentrated at interface of dermis with epidermis.
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Specimen
1h:
postnatal day 1
(more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Specimen
1h:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
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Specimen
1i:
postnatal day 1
(more )
Note: Double labeled: green - Crabp1; red - Mki67 (assay MGI:6196083).
Specimen
1i:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 1
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Crabp1; red - Mki67 (assay MGI:6196083).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: back skin |
Weak |
Regionally restricted |
1i
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Reduced intensity of expression relative to embryonic skin.
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TS27: dermis |
Present |
Regionally restricted |
1i
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Expression in dermal fibroblasts at dermal-epidermal interface.
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Specimen
1j:
postnatal day 2
(more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Specimen
1j:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 2
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
|
Structure |
Level |
Pattern |
Image |
Note |
TS27: dermis |
Weak |
Regionally restricted |
1j
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Intensity of expression in dermal fibroblasts was lower than at P1.
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Specimen
1l:
postnatal day 5
(more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Specimen
1l:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
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Specimen
1l inset:
postnatal day 5
(more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Specimen
1l inset:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
|
Structure |
Level |
Pattern |
Image |
Note |
TS28: back skin |
Present |
Regionally restricted |
1l inset
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Expression in small population of cells located beneath the developing sebaceous gland, whose morphology differed from that of surrounding dermal fibroblasts.
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Specimen
1n:
postnatal day 42
(more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Specimen
1n:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 42
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
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Specimen
1o:
postnatal day 102
(more )
Note: Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
Specimen
1o:
(close )
Genetic Background:
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involves: C57BL/6 * CBA
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Age:
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postnatal day 102
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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Formalin
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Crabp1; red - Krt14 (assay MGI:6196081).
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