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Gene Expression Data
Assay Details
Assay
Reference: J:260419 Jiang X, et al., Asymmetric distribution of CRUMBS polarity complex proteins from compacted 8-cell to blastocyst stage during mouse preimplantation development. Gene Expr Patterns. 2018 Jan;27:93-98
Assay type: Immunohistochemistry
MGI Accession ID: MGI:6192270
Gene symbol: Pals1
Gene name: protein associated with LIN7 1, MAGUK family member
Antibody: anti-PALS1 (sc-33831)
Detection system: Secondary antibody coupled to fluorescein
Assay notes: Confocal images.
Results
Specimen 3 c8c: embryonic day 2.5 (more )
Note: Compacted 8-cell embryo.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Present Regionally restricted 3_c8c , 3_c8c Merge Expression is at the apical membrane.
Specimen 3 Mo: embryonic day 3.0 (more )
Note: Morula stage embryo.
Structure Level Pattern Image Note
TS3: compacted morula Present Regionally restricted 3_Mo , 3_Mo Merge Expression is at the apical membrane. Very weak expression is found at the basolateral membrane.
Specimen 3 Bl: embryonic day 3.5 (more )
Note: Blastocyst stage embryo.
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted 3_Bl Merge , 3_Bl Expression is at the apical membrane. Very weak expression is found at the basolateral membrane.
Specimen 6B: embryonic day 3.5 (more )
Note: Blastocyst stage embryo. Double labeled: green - Pals1; red - Cdh1 (assay MGI:6192262).
Structure Level Pattern Image Note
TS4: inner cell mass Present Regionally restricted 6B PALS1 , 6B Merge Expression is at the apical membrane.
Specimen S3 Zy: embryonic day 0.5 (more )
Note: Zygote.
Structure Level Pattern Image Note
TS1: 1-cell stage embryo Weak Uniform S3_Zy Merge , S3_Zy Expression is in the cytoplasm.
Specimen S3 2c: embryonic day 1.0 (more )
Note: 2-cell stage embryo.
Structure Level Pattern Image Note
TS2: 2-cell stage embryo Weak Uniform S3_2c , S3_2c Merge Expression is in the cytoplasm.
Specimen S3 4c: embryonic day 2.0 (more )
Note: 4-cell stage embryo.
Structure Level Pattern Image Note
TS3: 4-cell stage embryo Weak Uniform S3_4c , S3_4c Merge Expression is in the cytoplasm.
Specimen S3 8c: embryonic day 2.0 (more )
Note: 8-cell stage embryo.
Structure Level Pattern Image Note
TS3: 8-cell stage embryo Weak Uniform S3_8c Merge , S3_8c Expression is in the cytoplasm.
Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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05/21/2024
MGI 6.23 		The Jackson Laboratory