Gene Expression Data
Assay Details
Assay
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Reference:
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J:231047
Rios-Rojas C, et al., Germ cells influence cord formation and leydig cell gene expression during mouse testis development. Dev Dyn. 2016 Apr;245(4):433-44
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6154707
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Gene symbol:
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Gata4
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Gene name:
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GATA binding protein 4
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Results
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Specimen
1B Control:
embryonic day 16.5
(more )
Note: Double labeled: green - Gata4; red - Ddx4 (assay MGI:6154706).
Specimen
1B Control:
(close )
Genetic Background:
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Not Specified
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
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Note:
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Double labeled: green - Gata4; red - Ddx4 (assay MGI:6154706).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
1B Control
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Expression in nuclei of Sertoli cells.
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Specimen
1B We:
embryonic day 16.5; KitW-e/ KitW-e
(more )
Note: Double labeled: green - Gata4; red - Ddx4 (assay MGI:6154706).
Specimen
1B We:
(close )
Genetic Background:
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involves: 101 * C3H * Quackenbush Swiss
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Mutant Allele(s):
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KitW-e/KitW-e
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
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Paraffin
|
Note:
|
Double labeled: green - Gata4; red - Ddx4 (assay MGI:6154706).
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
1B We
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Expression in Sertoli cells whose nuclei form a circular outline in cross sections.
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Specimen
4B Control:
embryonic day 16.5
(more )
Specimen
4B Control:
(close )
Genetic Background:
|
Not Specified
|
Age:
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embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Specimen
4B We:
(close )
Genetic Background:
|
involves: 101 * C3H * Quackenbush Swiss
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Mutant Allele(s):
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KitW-e/KitW-e
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Age:
|
embryonic day 16.5
|
Sex:
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Not Specified
|
Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Structure |
Cell Type |
Level |
Pattern |
Image |
Note |
TS24: Sertoli cell |
Sertoli cell |
Present |
Regionally restricted |
4B We
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Authors report number of expressing cells was reduced in mutant compared with wild-type.
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Specimen
5A Control:
embryonic day 16.5
(more )
Note: Double labeled: green - Gata4; red - Casp3 (assay MGI:6154710).
Specimen
5A Control:
(close )
Genetic Background:
|
Not Specified
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Paraffin
|
Note:
|
Double labeled: green - Gata4; red - Casp3 (assay MGI:6154710).
|
Specimen
5A We:
embryonic day 16.5; KitW-e/ KitW-e
(more )
Note: Double labeled: green - Gata4; red - Casp3 (assay MGI:6154710).
Specimen
5A We:
(close )
Genetic Background:
|
involves: 101 * C3H * Quackenbush Swiss
|
Mutant Allele(s):
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KitW-e/KitW-e
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Age:
|
embryonic day 16.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
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4% Paraformaldehyde
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Embedding:
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Paraffin
|
Note:
|
Double labeled: green - Gata4; red - Casp3 (assay MGI:6154710).
|
|