Gene Expression Data
Assay Details
Assay
|
Reference:
|
J:180189
Mori N, et al., Ccdc85c encoding a protein at apical junctions of radial glia is disrupted in hemorrhagic hydrocephalus (hhy) mice. Am J Pathol. 2012 Jan;180(1):314-27
|
Assay type:
|
Immunohistochemistry
|
MGI Accession ID:
|
MGI:6153960
|
Gene symbol:
|
Ccdc85c
|
Gene name:
|
coiled-coil domain containing 85C
|
Antibody:
|
Ccdc85C antibody
|
Detection system:
|
Secondary antibody coupled to Alexa Fluor 488
|
Assay notes:
|
Specimens were fixed in Zamboni's fixative containing 0.21% picric acid and 2% paraformaldehyde.
|
|
Results
|
Specimen
3A:
embryonic day 18.0
(more )
Specimen
3A:
(close )
Genetic Background:
|
involves: BALB/cHeA * MSM
|
Age:
|
embryonic day 18.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Specimen
3B:
embryonic day 15.0
(more )
Specimen
3B:
(close )
Genetic Background:
|
involves: BALB/cHeA * MSM
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Specimen
3C:
(close )
Genetic Background:
|
involves: BALB/cHeA * MSM
|
Mutant Allele(s):
|
Ccdc85chhy/Ccdc85chhy
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: brain |
Absent |
|
3C
|
|
Specimen
3D:
postnatal day 0
(more )
Specimen
3D:
(close )
Genetic Background:
|
involves: BALB/cHeA * MSM
|
Age:
|
postnatal day 0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Specimen
3E:
postnatal day 5
(more )
Specimen
3E:
(close )
Genetic Background:
|
involves: BALB/cHeA * MSM
|
Age:
|
postnatal day 5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Specimen
3G:
embryonic day 15.0
(more )
Note: Double labeled: green - Ccdc85c; red - Tjp1 (assay MGI:6153967).
Specimen
3G:
(close )
Genetic Background:
|
involves: BALB/cHeA * MSM
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Ccdc85c; red - Tjp1 (assay MGI:6153967).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: telencephalon |
Present |
Regionally restricted |
3H
,
3G
|
Expression is in adherens junctions.
|
Specimen
3J:
embryonic day 15.0
(more )
Note: Double labeled: green - Ccdc85c; red - Tjp1 (assay MGI:6153967).
Specimen
3J:
(close )
Genetic Background:
|
involves: BALB/cHeA * MSM
|
Age:
|
embryonic day 15.0
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
Other - See Notes
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Ccdc85c; red - Tjp1 (assay MGI:6153967).
|
Structure |
Level |
Pattern |
Image |
Note |
TS23: telencephalon |
Present |
Regionally restricted |
3K
,
3J
|
Expression is in apical junctions in radial glial cells.
|
|