Gene Expression Data
Assay Details
Assay
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Reference:
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J:227089
Pirson M, et al., Expression of peroxiredoxins and thioredoxins in the mouse spinal cord during embryonic development. J Comp Neurol. 2015 Dec 1;523(17):2599-617
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Assay type:
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Immunohistochemistry
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MGI Accession ID:
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MGI:6144139
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Gene symbol:
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Prdx5
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Gene name:
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peroxiredoxin 5
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Antibody:
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Prdx5 antibody
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Detection system:
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Secondary antibody coupled to Alexa Fluor 488
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Results
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Specimen
2F/H:
embryonic day 16.5
(more )
Note: Double labeled: green - Prdx5; red - Atp1b1 (assay MGI:6144143).
Specimen
2F/H:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 16.5
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Sex:
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Not Specified
|
Type:
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section
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Fixation:
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4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Prdx5; red - Atp1b1 (assay MGI:6144143).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spinal cord |
Present |
Punctate |
2F
,
2H
|
Authors report punctate expression and co-localization with mitochondrial marker, Atpb.
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Specimen
2J/K:
embryonic day 16.5
(more )
Note: Double labeled: green - Prdx5; red - Cat (assay MGI:6144144).
Specimen
2J/K:
(close )
Genetic Background:
|
C57BL/6N
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Age:
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embryonic day 16.5
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Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Double labeled: green - Prdx5; red - Cat (assay MGI:6144144).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spinal cord |
Weak |
Regionally restricted |
2K
,
2J
|
Authors report weak expression in cytosol but little overlap with peroxisomal marker, Cat.
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Specimen
7A:
embryonic day 10.5
(more )
Specimen
7A:
(close )
Genetic Background:
|
C57BL/6N
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Age:
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embryonic day 10.5
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Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS17: future spinal cord |
Ambiguous |
|
7A
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Authors describe expression at E10.5 as very low or even absent.
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Specimen
7B:
embryonic day 11.5
(more )
Specimen
7B:
(close )
Genetic Background:
|
C57BL/6N
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Age:
|
embryonic day 11.5
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Sex:
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Not Specified
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Type:
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section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS19: future spinal cord |
Strong |
Regionally restricted |
7B
|
Strong expression in ventral half of spinal cord.
|
Specimen
7C:
embryonic day 12.5
(more )
Specimen
7C:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 12.5
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Sex:
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Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Strong |
Regionally restricted |
7C
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Intense expression in cells located ventrally and laterally in spinal cord. Increased expression in isolated cells in the vicinity of the sensory nerve root.
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Specimen
7D:
embryonic day 13.5
(more )
Specimen
7D:
(close )
Genetic Background:
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C57BL/6N
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Age:
|
embryonic day 13.5
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Sex:
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Not Specified
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Type:
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section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Structure |
Level |
Pattern |
Image |
Note |
TS21: spinal cord |
Strong |
Single cells |
7D
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Increased expression in isolated cells in the vicinity of the sensory nerve root.
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Specimen
7E:
embryonic day 14.5
(more )
Specimen
7E:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 14.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
7F:
embryonic day 16.5
(more )
Specimen
7F:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
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Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
7G:
embryonic day 18.5
(more )
Specimen
7G:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 18.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Specimen
7H/K:
embryonic day 12.5
(more )
Note: Triple labeled: green - Prdx5; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
Specimen
7H/K:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Prdx5; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Strong |
Regionally restricted |
7H
,
7K
|
Authors report strong ventral expression co-localized in nucleus with motor neuron markers Isl1/2 and Foxp1.
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Specimen
7L/O:
embryonic day 12.5
(more )
Note: Triple labeled: green - Prdx5; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
Specimen
7L/O:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 12.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Prdx5; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
|
Structure |
Level |
Pattern |
Image |
Note |
TS20: spinal cord |
Present |
Punctate |
7L
,
7O
|
Punctuated pattern of expression typical of mitochondrial localization.
|
Specimen
7P/S:
embryonic day 16.5
(more )
Note: Triple labeled: green - Prdx5; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
Specimen
7P/S:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Prdx5; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spinal cord |
Strong |
Regionally restricted |
7P
,
7S
|
Authors report strong ventral expression co-localized in nucleus with motor neuron markers Isl1/2 and Foxp1.
|
Specimen
7T/W:
embryonic day 16.5
(more )
Note: Triple labeled: green - Prdx5; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
Specimen
7T/W:
(close )
Genetic Background:
|
C57BL/6N
|
Age:
|
embryonic day 16.5
|
Sex:
|
Not Specified
|
Type:
|
section
|
Fixation:
|
4% Paraformaldehyde
|
Embedding:
|
Cryosection
|
Note:
|
Triple labeled: green - Prdx5; purple - Foxp1 (assay MGI:6144147); red - Isl1 (assay MGI:6144145) and Isl2 (assay MGI:6144251).
|
Structure |
Level |
Pattern |
Image |
Note |
TS24: spinal cord |
Present |
Punctate |
7T
,
7W
|
Punctuated pattern of expression typical of mitochondrial localization.
|
|